2026-04-19T16:16:26Zhttps://recercat.cat/oai/request

oai:recercat.cat:10230/718122025-11-08T14:43:24Zcom_2072_6col_2072_452952

00925njm 22002777a 4500 dc Melo-Ximenes, Amanda Alves de author Batista, Romina author Corvalán, Leonardo Carlos Jeronimo author Marquès i Bonet, Tomàs, 1975- author Kuderna, Lukas, 1989- author Farh, Kyle author Rogers, Jeffrey author Kaizer, Mariane da Cruz author Boubli, Jean P. author Melo, Fabiano Rodrigues de author Nunes, Rhewter author Telles, Mariana Pires de Campos author 2025-11-07T15:24:04Z 2025-11-07T15:24:04Z 2025 2025-11-07T15:24:04Z Genomic resources, such as draft genomes, are vital for biodiversity monitoring and conservation. For endangered species, they enable the development of tools like organellar genomes and molecular markers, which are crucial for population genetics. Advances in sequencing technologies now allow high-throughput genotyping with detailed amplicon sequences, enhancing genetic variation studies. The northern muriqui (Brachyteles hypoxanthus), a critically endangered primate endemic to Brazil's Atlantic Forest, currently lacks both nuclear and mitochondrial genome data and species-specific microsatellite markers for population genetic studies. We assembled a 2.52 Gb draft genome for B. hypoxanthus with 202,243 contigs (N50 = 29,134 bp), and BUSCO analyses indicated 52% completeness and 15.5% fragmented genes. The complete 16,635 bp mitochondrial genome retains the conserved mammalian structure with 22 tRNAs, 2 rRNAs, 13 CDS, and an origin of replication. Additionally, we designed 31 SSR primer pairs suitable for non-invasive sampling and genotyping, alongside two mtDNA and two sex-determination primers, configured into three multiplex PCR sets. These genomic resources, including the draft genome, complete mitochondrial genome, and microsatellite markers, provide essential tools for evolutionary analyses and the genetic monitoring of B. hypoxanthus populations, supporting its conservation. http://hdl.handle.net/10230/71812 Conservation genomics GBS Genomics HTS SSR SSR-Seq First assembly of a draft genome of the critically endangered northern muriqui (Brachyteles hypoxanthus, primates, atelidae) including non-invasive genotyping strategies for the species