2026-04-13T07:36:16Zhttps://recercat.cat/oai/requestoai:recercat.cat:10230/717802025-11-08T15:00:35Zcom_2072_6col_2072_452952
A straightforward preparation of aminoglycoside-dinucleotide and -diPNA conjugates via click ligation assisted by microwaves
Alguacil, Javier
Defaus, Sira
Claudio, Ana
Trapote, Alejandro
Masides, Marta
Robles, Jordi
Aminoglycoside
Nucleic acids
Conjugate
Cycloaddition
Microwaves
An alternative and straightforward method to prepare aminoglycoside-dinucleotide and -diPNA conjugates is reported, which is based on copper-catalyzed Huisgen azide-alkyne cycloaddition ("click chemistry" ligation) assisted by microwave irradiation. This method permitted conjugations to be performed in aqueous solution, in very short times and with readily prepared precursors.
This work was supported by funds from the Spanish Ministerio de Educación y Ciencia (CTQ2007-68014-C02-01/BQU) and the Generalitat de Catalunya (2005SGR00693). J. A. and A. C. acknowledge predoctoral fellowships from the University of Barcelona (BRD and IBUB programs). The authors also wish to acknowledge the Mass Spectrometry (Dr. Irene Fernández and and Laura Ortiz) and NMR facilities (Drs. Ma. Antònia Molins
and Francisco Cárdenas) of the Scientific-Technical Services of the University of Barcelona for technical support, and Ana Soriano for his help with the preparation of di(p-nitrophenyl) carbonate and its use in the synthesis of alkynyl-diPNAs.
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2010
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info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://hdl.handle.net/10230/71780
European Journal of Organic Chemistry. 2010;16:3102-9
info:eu-repo/grantAgreement/ES/2PN/CTQ2007-68014-C02-01
This is the peer reviewed version of the following article: Alguacil J, Defaus S, Claudio A, Trapote A, Masides M, Robles J. A straightforward preparation of aminoglycoside-dinucleotide and -diPNA conjugates via click ligation assisted by microwaves. European Journal Of Organic Chemistry. 2010;16:3102-9. DOI: 10.1002/ejoc.201000182, which has been published in final form at http://dx.doi.org/10.1002/ejoc.201000182. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
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