2026-04-17T07:43:28Zhttps://recercat.cat/oai/request

oai:recercat.cat:10230/717802025-11-08T15:00:35Zcom_2072_6col_2072_452952

00925njm 22002777a 4500 dc Alguacil, Javier author Defaus, Sira author Claudio, Ana author Trapote, Alejandro author Masides, Marta author Robles, Jordi author 2025-11-05T13:58:44Z 2025-11-05T13:58:44Z 2010 2025-11-05T13:58:43Z An alternative and straightforward method to prepare aminoglycoside-dinucleotide and -diPNA conjugates is reported, which is based on copper-catalyzed Huisgen azide-alkyne cycloaddition ("click chemistry" ligation) assisted by microwave irradiation. This method permitted conjugations to be performed in aqueous solution, in very short times and with readily prepared precursors. This work was supported by funds from the Spanish Ministerio de Educación y Ciencia (CTQ2007-68014-C02-01/BQU) and the Generalitat de Catalunya (2005SGR00693). J. A. and A. C. acknowledge predoctoral fellowships from the University of Barcelona (BRD and IBUB programs). The authors also wish to acknowledge the Mass Spectrometry (Dr. Irene Fernández and and Laura Ortiz) and NMR facilities (Drs. Ma. Antònia Molins and Francisco Cárdenas) of the Scientific-Technical Services of the University of Barcelona for technical support, and Ana Soriano for his help with the preparation of di(p-nitrophenyl) carbonate and its use in the synthesis of alkynyl-diPNAs. http://hdl.handle.net/10230/71780 Aminoglycoside Nucleic acids Conjugate Cycloaddition Microwaves A straightforward preparation of aminoglycoside-dinucleotide and -diPNA conjugates via click ligation assisted by microwaves