2026-04-17T22:04:59Zhttps://recercat.cat/oai/request

oai:recercat.cat:10230/597762025-12-22T13:33:54Zcom_2072_6col_2072_452952

00925njm 22002777a 4500 dc Lázaro, Iolanda author Bobi, Joaquim author Cofán, Montserrat author Kapravelou, Garyfallia author Amor, Antonio J. author Surra, Joaquim author Gómez Guerrero, Carmen author Ortega, Emilio author Osada, Jesús author Dantas, Ana Paula author Sala Vila, Aleix author 2024-04-15T10:15:23Z 2024-04-15T10:15:23Z 2023 Introduction: The lower rates of cardiovascular disease in Southern Europe could be partially explained by the low prevalence of lipid-rich atheroma plaques. Consumption of certain foods affects the progression and severity of atherosclerosis. We investigated whether the isocaloric inclusion of walnuts within an atherogenic diet prevents phenotypes predicting unstable atheroma plaque in a mouse model of accelerated atherosclerosis. Methods: Apolipoprotein E-deficient male mice (10-week-old) were randomized to receive a control diet (9.6% of energy as fat, n = 14), a palm oil-based high-fat diet (43% of energy as fat, n = 15), or an isocaloric diet in which part of palm oil was replaced by walnuts in a dose equivalent to 30 g/day in humans (n = 14). All diets contained 0.2% cholesterol. Results: After 15 weeks of intervention, there were no differences in size and extension in aortic atherosclerosis among groups. Compared to control diet, palm oil-diet induced features predicting unstable atheroma plaque (higher lipid content, necrosis, and calcification), and more advanced lesions (Stary score). Walnut inclusion attenuated these features. Palm oil-based diet also boosted inflammatory aortic storm (increased expression of chemokines, cytokines, inflammasome components, and M1 macrophage phenotype markers) and promoted defective efferocytosis. Such response was not observed in the walnut group. The walnut group’s differential activation of nuclear factor kappa B (NF-κB; downregulated) and Nrf2 (upregulated) in the atherosclerotic lesion could explain these findings. Conclusion: The isocaloric inclusion of walnuts in an unhealthy high-fat diet promotes traits predicting stable advanced atheroma plaque in mid-life mice. This contributes novel evidence for the benefits of walnuts, even in an unhealthy dietary environment. AS-V was supported by the CWC and the Instituto de Salud Carlos III (ISCIII-FIS-FEDER grant PI15/01014). CG-G is supported by the Spanish Ministry of Science and Innovation (PID2021-127741OB-I00). The funding agencies had no involvement in the study design, data collection, analyses, interpretation of the data, or writing of the manuscript. Palm oil Unsaturated fat Nuts Nrf2 Inflammation Efferocytosis Walnut inclusion in a palm oil-based atherogenic diet promotes traits predicting stable atheroma plaque in Apoe-deficient mice