2026-04-17T20:43:14Zhttps://recercat.cat/oai/request

oai:recercat.cat:10230/593222026-03-18T23:46:54Zcom_2072_6col_2072_452952

00925njm 22002777a 4500 dc Sans-de San Nicolás, Lídia author Figueras-Nart, Ignasi author García-Jiménez, Irene author Bonfill-Ortí, Montserrat author Guilabert, Antonio author Curto Barredo, Laia author Bertolín Colilla, Marta author Ferran Farrés, Marta author Serra-Baldrich, Esther author Pujol Vallverdú, Ramón M. author Santamaria-Babí, Luis F. author 2024-03-05T07:32:26Z 2024-03-05T07:32:26Z 2023 Background: The role of allergen sensitization in IL-31 production by T cells and specifically in the clinical context of atopic dermatitis (AD) has not been characterized. Methods: The response to house dust mite (HDM) in purified memory T cells cocultured with epidermal cells from AD patients (n=58) and control subjects (n=11) was evaluated. AD-associated cytokines from culture supernatants, plasma proteins and mRNA expression from cutaneous lesions were assessed and related with the clinical features of the patients. Results: HDM-induced IL-31 production by memory T cells defined two subsets of AD patients according to the presence or absence of IL-31 response. Patients in the IL-31 producing group showed a more inflammatory profile, and increased HDM-specific (sp) and total IgE levels compared to the IL-31 non-producing group. A correlation between IL-31 production and patient's pruritus intensity, plasma CCL27 and periostin was detected. When the same patients were analyzed based on sp IgE and total IgE levels, an increased IL-31 in vitro response, as well as type 2 markers in plasma and cutaneous lesions, was found in patients with sp IgE levels > 100 kUA/L and total IgE levels > 1000 kU/L. The IL-31 response by memory T cells was restricted to the cutaneous lymphocyte-associated antigen (CLA)+ T-cell subset. Conclusion: IgE sensitization to HDM allows stratifying IL-31 production by memory T cells in AD patients and relating it to particular clinical phenotypes of the disease. CCL27 CLA+ memory T cells IL-31 IgE Atopic dermatitis House dust mite Periostin Pruritus Allergen sensitization stratifies IL-31 production by memory T cells in atopic dermatitis patients