2026-04-13T02:30:11Zhttps://recercat.cat/oai/request

oai:recercat.cat:10230/588462025-12-18T01:07:30Zcom_2072_6col_2072_452952

00925njm 22002777a 4500 dc Kharraz, Yacine author Lefort, Anne author Libert, Frédérick author Mann, Christopher J. author Gueydan, Cyril author Kruys, Véronique author 2024-01-29T07:20:43Z 2024-01-29T07:20:43Z 2016 TIA-1 related protein (TIAR) is a RNA-binding protein involved in several steps of gene expression such as RNA splicing Aznarez et al. (2008) [1] and translation Piecyk et al. (2000) [2]. TIAR contains three RNA recognition motifs (RRMs) allowing its interaction with specific sequences localized in the untranslated regions (UTRs) of several mRNAs. In myeloid cells, TIAR has been shown to bind and regulate the translation and stability of various mRNA-encoding proteins important for the inflammatory response, such as TNFα Piecyk et al. (2000), Gueydan et al. (1999) [2], [3], Cox-2 Cok et al. (2003) [4] or IL-8 Suswam et al. (2005) [5]. Here, we generated two macrophage-like RAW 264.7 cell lines expressing either a tagged full-length TIAR protein or a RRM2-truncated mutant unable to bind RNA with high affinity Dember et al. (1996), Kim et al. (2013) . By a combination of RNA-IP and microarray analysis (RIP-chip), we identified mRNAs specifically bound by the full-length protein both in basal conditions and in response to LPS (GSE77577). AU-rich element LPS RIP-chip TIAR Translation repression Genome-wide analysis of TIAR RNA ligands in mouse macrophages before and after LPS stimulation