2026-04-18T03:20:53Zhttps://recercat.cat/oai/request

oai:recercat.cat:10230/550942025-12-18T01:23:57Zcom_2072_6col_2072_452952

00925njm 22002777a 4500 dc Rubio Pérez, Miguel Angel author Herrando Grabulosa, Mireia author Gaja-Capdevila, Nuria author Vilches, Jorge J. author Navarro, Xavier author 2022-12-09T08:04:58Z 2022-12-09T08:04:58Z 2022 SOD1G93A mice show loss of cutaneous small fibers, as in ALS patients. Our objective is to characterize the involvement of different somatosensory neuron populations and its temporal progression in the SOD1G93A mice. We aim to further define peripheral sensory involvement, analyzing at the same time points the neuronal bodies located in the dorsal root ganglia (DRG) and the distal part of their axons in the skin, in order to shed light in the mechanisms of sensory involvement in ALS. We performed immunohistochemical analysis of peptidergic (CGRP), non-peptidergic (IB4) fibers in epidermis, as well as sympathetic sudomotor fibers (VIP) in the footpads of SOD1G93A mice and wild type littermates at 4, 8, 12 and 16 weeks of age. We also immunolabeled and quantified neuronal bodies of IB4, CGRP and parvalbumin (PV) positive sensory neurons in lumbar DRG. We detected a reduction of intraepidermal nerve fiber density in the SOD1G93A mice of both peptidergic and non-peptidergic axons, compared with the WT, being the non-peptidergic the fewest. Sweat gland innervation was similarly affected in the SOD1G93A mouse at 12 weeks. Nonetheless, the number of DRG neurons from different sensory populations remained unchanged during all stages. Cutaneous sensory axons are affected in the SOD1G93A mouse, with non-peptidergic being slightly more vulnerable than peptidergic axons. Loss or lack of growth of the distal portion of sensory axons with preservation of the corresponding neuronal bodies suggest a distal axonopathy. Esclerosi lateral amiotròfica Animals Rates (Animals de laboratori) Neurones sensorials Characterization of somatosensory neuron involvement in the SOD1G93A mouse model