2026-04-17T14:06:22Zhttps://recercat.cat/oai/requestoai:recercat.cat:10230/545912025-12-12T01:52:10Zcom_2072_6col_2072_452952
RECERCAT
author
Dalmasso, Giovanni
author
Musy, Marco
author
Niksic, Martina
author
Robert Moreno, Alexandre
author
Badía Careaga, Claudio
author
Sanz-Ezquerro, Juan José
author
Sharpe, James
2022-10-26T06:25:37Z2022-10-26T06:25:37Z2022
Normal organogenesis cannot be recapitulated in vitro for mammalian organs, unlike in species including Drosophila and zebrafish. Available 3D data in the form of ex vivo images only provide discrete snapshots of the development of an organ morphology. Here, we propose a computer-based approach to recreate its continuous evolution in time and space from a set of 3D volumetric images. Our method is based on the remapping of shape data into the space of the coefficients of a spherical harmonics expansion where a smooth interpolation over time is simpler. We tested our approach on mouse limb buds and embryonic hearts. A key advantage of this method is that the resulting 4D trajectory can take advantage of all the available data while also being able to interpolate well through time intervals for which there are little or no data. This allows for a quantitative, data-driven 4D description of mouse limb morphogenesis.This research was supported by core funding from EMBL and CRG, and also the ERC advanced grant SIMBIONT (project no. 670555) and a grant from the Fundacion La Marato TV3 (grant 082030). G.D. and M.M. were supported by the ERC advanced grant SIMBIONT (project no. 670555).
© 2022 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/) http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess
OPT
Heart growth
Limb growth
Morphogenesis
Mouse development
Spherical harmonics
Voxel data
4D reconstruction of murine developmental trajectories using spherical harmonics
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion