2026-04-17T03:18:08Zhttps://recercat.cat/oai/request

oai:recercat.cat:10230/545912025-12-12T01:52:10Zcom_2072_6col_2072_452952

00925njm 22002777a 4500 dc Dalmasso, Giovanni author Musy, Marco author Niksic, Martina author Robert Moreno, Alexandre author Badía Careaga, Claudio author Sanz-Ezquerro, Juan José author Sharpe, James author 2022-10-26T06:25:37Z 2022-10-26T06:25:37Z 2022 Normal organogenesis cannot be recapitulated in vitro for mammalian organs, unlike in species including Drosophila and zebrafish. Available 3D data in the form of ex vivo images only provide discrete snapshots of the development of an organ morphology. Here, we propose a computer-based approach to recreate its continuous evolution in time and space from a set of 3D volumetric images. Our method is based on the remapping of shape data into the space of the coefficients of a spherical harmonics expansion where a smooth interpolation over time is simpler. We tested our approach on mouse limb buds and embryonic hearts. A key advantage of this method is that the resulting 4D trajectory can take advantage of all the available data while also being able to interpolate well through time intervals for which there are little or no data. This allows for a quantitative, data-driven 4D description of mouse limb morphogenesis. This research was supported by core funding from EMBL and CRG, and also the ERC advanced grant SIMBIONT (project no. 670555) and a grant from the Fundacion La Marato TV3 (grant 082030). G.D. and M.M. were supported by the ERC advanced grant SIMBIONT (project no. 670555). OPT Heart growth Limb growth Morphogenesis Mouse development Spherical harmonics Voxel data 4D reconstruction of murine developmental trajectories using spherical harmonics