2026-04-14T04:19:36Zhttps://recercat.cat/oai/request

oai:recercat.cat:10230/467182025-12-20T16:52:50Zcom_2072_6col_2072_452952

00925njm 22002777a 4500 dc Chen, Tzu-Chun author Talló Parra, Marc, 1992- author Cao, Qian M. author Kadener, Sebastian author Böttcher, René author Pérez Vilaró, Gemma, 1985- author Boonchuen, Pakpoom author Somboonwiwat, Kunlaya author Díez Antón, Juana, 1962- author Sarnow, Peter author 2021-03-10T11:08:50Z 2021-03-10T11:08:50Z 2020 Viruses subvert macromolecular pathways in infected host cells to aid in viral gene amplification or to counteract innate immune responses. Roles for host-encoded, noncoding RNAs, including microRNAs, have been found to provide pro- and anti-viral functions. Recently, circular RNAs (circRNAs), that are generated by a nuclear back-splicing mechanism of pre-mRNAs, have been implicated to have roles in DNA virus-infected cells. This study examines the circular RNA landscape in uninfected and hepatitis C virus (HCV)-infected liver cells. Results showed that the abundances of distinct classes of circRNAs were up-regulated or down-regulated in infected cells. Identified circRNAs displayed pro-viral effects. One particular up-regulated circRNA, circPSD3, displayed a very pronounced effect on viral RNA abundances in both hepatitis C virus- and Dengue virus-infected cells. Though circPSD3 has been shown to bind factor eIF4A3 that modulates the cellular nonsense-mediated decay (NMD) pathway, circPSD3 regulates RNA amplification in a pro-viral manner at a post-translational step, while eIF4A3 exhibits the anti-viral property of the NMD pathway. Findings from the global analyses of the circular RNA landscape argue that pro-, and likely, anti-viral functions are executed by circRNAs that modulate viral gene expression as well as host pathways. Because of their long half-lives, circRNAs likely play hitherto unknown, important roles in viral pathogenesis. This study was funded by the National Institutes of Health (https://www.nih.gov) (R01AI06900011) and NIH grants R01GM122406 and R01AG057700 to S.K. Funds for J.D. were received from the Spanish Ministry of Economy and Competitiveness (AEI/ MINECO/FEDER,UE) through grants BFU2016–80039-R and Unidad de Excelencia Maria de Maeztu funded by the MINECO (ref: MDM-2014–0370), and by a 2017 SGR 909 grant by the Secretaria d’Universitats i Recerca del Departament d’Economia i Coneixement of the Generalitat de Catalunya Virus de l'hepatitis C Genètica Fetge Host-derived circular RNAs display proviral activities in Hepatitis C virus-infected cells