2026-04-13T05:56:17Zhttps://recercat.cat/oai/request

oai:recercat.cat:10230/462462025-12-24T08:35:04Zcom_2072_6col_2072_452952

00925njm 22002777a 4500 dc Ghorbel, Amina author André, Franck M. author Mir, Lluis M. author García Sánchez, Tomás author 2021-01-22T09:03:48Z 2021 The use of conductive nanoparticles (NPs) was previously proposed as a way to locally amplify the electric field (EF) intensity at the cell membrane to enhance cell electroporation. To achieve this, a close distance between the NPs and the cell membrane is mandatory. Here, a new method to improve the contact between NPs and cell surface using the effects of electric pulses (electrophoretic forces) is explored. The effects of two types of electric pulses are analyzed alone or combined in a two-pulse-train protocol on Chinese hamster DC-3F cells. Particularly we used 100 µs duration pulses, low intensity-millisecond pulses and combinations of both. Finally, we studied the use of surface coated NPs (PEGylated) for this application. Our results demonstrate that the delivery of an electric field prior to the electroporation pulses increases the accumulation of NPs around the cell membrane suggesting that NPs are pushed towards the cell surface through electrophoretic forces. This allowed reducing the need for long incubations between cells and NPs to observe an enhancement of electroporation mediated by conductive NPs. Thus low intensity-millisecond pulses can be used to increase the accumulation of either aggregated or individual (i.e. PEGylated) NPs supporting the electrophoretic nature of the observed effects. This work was partially funded by La Ligue contre le Cancer postdoctoral fellowship program to TGS and a grant of Tunisian government to AG. The authors thank the funding support of the CNRS, Gustave Roussy, Univ. Paris-Sud and Université Paris-Saclay, as well as the ITMO Cancer in the frame of the Plan Cancer 2015-2019 (project PC201517). Gold nanoparticles Local electric field Pulsed electric fields Electroporation Electrophoresis-assisted accumulation of conductive nanoparticles for the enhancement of cell electropermeabilization