2026-04-17T10:48:30Zhttps://recercat.cat/oai/request

oai:recercat.cat:10230/422602025-12-12T03:02:25Zcom_2072_6col_2072_452952

00925njm 22002777a 4500 dc Schmiedel, Jörn M. author Carey, Lucas, 1980- author Lehner, Ben, 1978- author 2019-09-10T14:10:06Z 2019-09-10T14:10:06Z 2019 The effects of cell-to-cell variation (noise) in gene expression have proven difficult to quantify because of the mechanistic coupling of noise to mean expression. To independently quantify the effects of changes in mean expression and noise we determine the fitness landscapes in mean-noise expression space for 33 genes in yeast. For most genes, short-lived (noise) deviations away from the expression optimum are nearly as detrimental as sustained (mean) deviations. Fitness landscapes can be classified by a combination of each gene's sensitivity to protein shortage or surplus. We use this classification to explore evolutionary scenarios for gene expression and find that certain landscape topologies can break the mechanistic coupling of mean and noise, thus promoting independent optimization of both properties. These results demonstrate that noise is detrimental for many genes and reveal non-trivial consequences of mean-noise-fitness topologies for the evolution of gene expression systems. This work was supported by a European Research Council Consolidator grant (616434), the Spanish Ministry of Economy and Competitiveness (BFU2011–26206 and SEV-2012–0208), the AXA Research Fund, Agència de Gestió d’Ajuts Universitaris i de Recerca (AGAUR, 2014SGR831), FP7 project 4DCellFate (277899), the EMBL-CRG Systems Biology Program (all to B.L.), an EMBO Long-Term Fellowship (ALTF 857–2016), the European Union’s Horizon 2020 research and innovation programme (Marie Skłodowska-Curie grant agreement No 752809) (both to J.M.S.) an AGAUR grant (2014SGR0974) and a MINECO grant (BFU2015–68351-P) (both to L.B.C.). The authors acknowledge support from the Spanish Ministry of Economy, Industry and Competitiveness (MEIC) to the EMBL partnership, the Centro de Excelencia Severo Ochoa, and the CERCA Programme / Generalitat de Catalunya. Cellular noise Evolution Evolvability Gene expression Empirical mean-noise fitness landscapes reveal the fitness impact of gene expression noise