2026-04-13T07:19:45Zhttps://recercat.cat/oai/request

oai:recercat.cat:10230/361652025-12-18T01:21:03Zcom_2072_6col_2072_452952

00925njm 22002777a 4500 dc Aymoz, Delphine author Solé, Carme author Pierre, Jean-Jerrold author Schmitt, Marta author Nadal Clanchet, Eulàlia de author Posas Garriga, Francesc author Pelet, Serge author 2018-12-20T15:34:45Z 2018-12-20T15:34:45Z 2018 During development, morphogens provide extracellular cues allowing cells to select a specific fate by inducing complex transcriptional programs. The mating pathway in budding yeast offers simplified settings to understand this process. Pheromone secreted by the mating partner triggers the activity of a MAPK pathway, which results in the expression of hundreds of genes. Using a dynamic expression reporter, we quantified the kinetics of gene expression in single cells upon exogenous pheromone stimulation and in the physiological context of mating. In both conditions, we observed striking differences in the timing of induction of mating-responsive promoters. Biochemical analyses and generation of synthetic promoter variants demonstrated how the interplay between transcription factor binding and nucleosomes contributes to determine the kinetics of transcription in a simplified cell-fate decision system. Work in the Pelet laboratory is supported by the Swiss National Science Foundation (PP00P3_139121), SystemsX.ch, and the University of Lausanne. Work in the Posas and de Nadal laboratories is funded by grants from the Spanish Ministry of Economy and Competitiveness (BFU2015‐64437‐P and FEDER to FP; BFU2017‐85152‐P and FEDER to EN), the Catalan Government (2017 SGR 799), the Fundación Botín, and the Banco Santander through its Santander Universities Global Division to FP. FP is recipient of an ICREA Acadèmia (Generalitat de Catalunya). Gene expression MAPK pathway Single‐cell measurements Yeast mating Timing of gene expression in a cell-fate decision system