2026-04-14T03:33:56Zhttps://recercat.cat/oai/request

oai:recercat.cat:10230/255662025-12-21T18:05:55Zcom_2072_6col_2072_452952

Reversible cysteine oxidation in hydrogen peroxide sensing and signal transduction García Santamarina, Sarela, 1978- Boronat i Llop, Susanna, 1965- Hidalgo Hernando, Elena Oxigen actiu Proteòmica Redox biology H2O2 reactivity Cys oxidation H2O2 sensor Redox proteomics Pap1 Yap1 OxyR Nrf2 roGFP Activation of redox cascades through hydrogen peroxide-mediated reversible cysteine oxidation is a major mechanism for intracellular signaling. Understanding why some cysteine residues are specifically oxidized, in competition with other proximal cysteine residues and in the presence of strong redox buffers, is therefore crucial for understanding redox signaling. In this review, we explore the recent advances in thiol-redox chemistry linked to signaling. We describe the last findings in the field of redox sensors, those that are naturally present in different model organisms as well as those that have been engineered to quantify intracellular hydrogen peroxide concentrations. Finally, we provide a summary of the newest approaches developed to study reversible cysteine oxidation at the proteomic level. This work was supported by the Spanish Ministry of Science and Innovation (BFU2012-32045), PLAN E and FEDER, by the Spanish program Consolider-Ingenio 2010 Grant CSD 2007-0020, and by Grant SGR2009-195 from Generalitat de Catalunya (Spain) to E.H. E.H. is the recipient of an ICREA Academia Award (Generalitat de Catalunya). 2016-01-13T15:06:38Z 2016-01-13T15:06:38Z 2014 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Biochemistry. 2014;53(16):2560-80 info:eu-repo/grantAgreement/ES/3PN/BFU2012-32045 © American Chemical Society after peer review and technical editing by the publisher./nTo access the final edited and published work see http://pubs.acs.org/doi/abs/10.1021/bi401700f info:eu-repo/semantics/openAccess American Chemical Society