2026-04-17T04:09:09Zhttps://recercat.cat/oai/request

oai:recercat.cat:10230/254932025-12-24T09:01:10Zcom_2072_6col_2072_452952

00925njm 22002777a 4500 dc Kuchina, Anna author Espinar, Lorena author Cagatay, Tolga author Balbin, Alejandro O. author Zhang, Fang author Alvarado, Alma author García Ojalvo, Jordi author Süel, Gürol M. author 2015-12-21T15:48:51Z 2015-12-21T15:48:51Z 2011 Multipotent differentiation, where cells adopt one of several possible fates, occurs in diverse systems ranging from bacteria to mammals. This decision-making process is driven by multiple differentiation programs that operate simultaneously in the cell. How these programs interact to govern cell fate choice is poorly understood. To investigate this issue, we simultaneously measured activities of the competing sporulation and competence programs in single Bacillus subtilis cells. This approach revealed that these competing differentiation programs progress independently without cross-regulation before the decision point. Cells seem to arrive at a fate choice through differences in the relative timing between the two programs. To test this proposed dynamic mechanism, we altered the relative timing by engineering artificial cross-regulation between the sporulation and competence circuits. Results suggest a simple model that does not require a checkpoint or intricate cross-regulation before cellular decision-making. Rather, cell fate choice appears to be the outcome of a 'molecular race' between differentiation programs that compete in time, providing a simple dynamic mechanism for decision-making. Fongs -- Genètica Meiosi Regulació genètica Cellular decision-making Differentiation dynamics Interactions between signaling pathways Quantitative single-cell dynamics Temporal competition between differentiation programs determines cell fate choice