Autor/a:
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Rubio, Mercedes; Bustamante Pineda, Mariona; Hernández Ferrer, Carles; Fernández Orth, Dietmar; Pantano, Lorena; Sarria, Yaris; Piqué Borras, Maria; Vellve, Kilian; Agramunt, Silvia; Carreras, Ramon; Estivill, Xavier, 1955-; González, Juan Ramón; Mayor Aparicio, Alfredo Gabriel
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Abstract:
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Circulating small RNAs, including miRNAs but also isomiRs and
other RNA species, have the potential to be used as non-invasive
biomarkers for communicable and non-communicable diseases. This
study aims to characterize and compare small RNA profiles in
human biofluids. For this purpose, RNA was extracted from plasma
and breast milk samples from 15 healthy postpartum mothers.
Small RNA libraries were prepared with the NEBNext(R) small RNA
library preparation kit and sequenced in an Illumina HiSeq2000
platform. miRNAs, isomiRs and clusters of small RNAs were
annotated using seqBuster/seqCluster framework in 5 plasma and
10 milk samples that passed the initial quality control. The RNA
yield was 81 ng/mL [standard deviation (SD): 41] and 3985 ng/mL
(SD: 3767) for plasma and breast milk, respectively. Mean number
of good quality reads was 4.04 million (M) (40.01% of the reads)
in plasma and 12.5M (89.6%) in breast milk. One thousand one
hundred eighty two miRNAs, 12,084 isomiRs and 1,053 small RNA
clusters that included piwi-interfering RNAs (piRNAs), tRNAs,
small nucleolar RNAs (snoRNA) and small nuclear RNAs (snRNAs)
were detected. Samples grouped by biofluid, with 308 miRNAs,
1,790 isomiRs and 778 small RNA clusters differentially
detected. In summary, plasma and milk showed a different small
RNA profile. In both, miRNAs, piRNAs, tRNAs, snRNAs, and snoRNAs
were identified, confirming the presence of non-miRNA species in
plasma, and describing them for the first time in milk. |