Título:
|
Evaluation of the effect of polymorphism on G-quadruplex-ligand interaction by means of spectroscopic and chromatographic techniques
|
Autor/a:
|
Benito, S.; Ferrer, A.; Benabou, Sanae; Aviñó Andrés, Anna; Eritja i Casadellà, Ramon; Gargallo Gómez, Raimundo
|
Otros autores:
|
Universitat de Barcelona |
Abstract:
|
Guanine-rich sequences may fold into highly ordered structures known as G-quadruplexes. Apart from the monomeric G-quadruplex, these sequences may form multimeric structures that are not usually considered when studying interaction with ligands. This work studies the interaction of a ligand, crystal violet, with three guanine-rich DNA sequences with the capacity to form multimeric structures. These sequences correspond to short stretches found near the promoter regions of c-kit and SMARCA4 genes. Instrumental techniques (circular dichroism, molecular fluorescence, size-exclusion chromatography and electrospray ionization mass spectrometry) and multivariate data analysis were used for this purpose. The polymorphism of G-quadruplexes was characterized prior to the interaction studies. The ligand was shown to interact preferentially with the monomeric G-quadruplex; the binding stoichiometry was 1:1 and the binding constant was in the order of 105 M-1 for all three sequences. The results highlight the importance of DNA treatment prior to interaction studies |
Materia(s):
|
-Polimorfisme (Cristal·lografia) -Anàlisi multivariable -Lligands -G-estructures -Polymorphism (Crystallography) -Multivariate analysis -Ligands -G-structures |
Derechos:
|
cc-by-nc-nd (c) Elsevier B.V., 2018
http://creativecommons.org/licenses/by-nc-nd/3.0/es |
Tipo de documento:
|
Artículo Artículo - Versión aceptada |
Editor:
|
Elsevier B.V.
|
Compartir:
|
|