Title:
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Discovery and validation of new potential biomarkers for early detection of colon cancer
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Author:
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Solé Acha, Xavier; Crous Bou, Marta; Cordero Romera, David; Olivares, David; Guinó, Elisabet; Sanz Pamplona, Rebeca; Rodríguez Moranta, Francisco; Sanjuan, Xavier; Oca Burguete, Javier de; Salazar Soler, Ramón; Moreno Aguado, Víctor
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Abstract:
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Background: accurate detection of characteristic proteins secreted by colon cancer tumor cells in biological fluids could serve as a biomarker for the disease. The aim of the present study was to identify and validate new serum biomarkers and demonstrate their potential usefulness for early diagnosis of colon cancer. Methods: the study was organized in three sequential phases: 1) biomarker discovery, 2) technical and biological validation, and 3) proof of concept to test the potential clinical use of selected biomarkers. A prioritized subset of the differentially-expressed genes between tissue types (50 colon mucosa from cancer-free individuals and 100 normal-tumor pairs from colon cancer patients) was validated and further tested in a series of serum samples from 80 colon cancer cases, 23 patients with adenoma and 77 cancer-free controls.Results: in the discovery phase, 505 unique candidate biomarkers were identified, with highly significant results and high capacity to discriminate between the different tissue types. After a subsequent prioritization, all tested genes (N = 23) were successfully validated in tissue, and one of them, COL10A1, showed relevant differences in serum protein levels between controls, patients with adenoma (p = 0.0083) and colon cancer cases (p = 3.2e-6). Conclusion: we present a sequential process for the identification and further validation of biomarkers for early detection of colon cancer that identifies COL10A1 protein levels in serum as a potential diagnostic candidate to detect both adenoma lesions and tumor. Impact:the use of a cheap serum test for colon cancer screening should improve its participation rates and contribute to decrease the burden of this disease. |
Subject(s):
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-Marcadors tumorals -Marcadors bioquímics -Càncer colorectal -Diagnòstic -Tumor markers -Biochemical markers -Colorectal cancer -Diagnosis |
Rights:
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cc-by (c) Solé Acha, Xavier et al., 2014
http://creativecommons.org/licenses/by/3.0/es |
Document type:
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Article Article - Published version |
Published by:
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Public Library of Science (PLoS)
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