Title:
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Towards the cell-instructive bactericidal substrate: exploring the combination of nanotopographical features and integrin selective synthetic ligands
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Author:
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Fraioli, Roberta; Tsimbouri, Penelope M.; Fisher, Leanne E.; Nobbs, Angela H.; Su, Bo; Neubauer, Stefanie; Rechenmacher, Florian; Kessler, Horst; Ginebra Molins, Maria Pau; Dalby, Matthew J.; Manero Planella, José María; Mas Moruno, Carlos
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Other authors:
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Universitat Politècnica de Catalunya. Departament de Ciència dels Materials i Enginyeria Metal·lúrgica; Universitat Politècnica de Catalunya. BBT - Biomaterials, Biomecànica i Enginyeria de Teixits; Technische Universität München; University of Bristol. Bristol Dental School |
Abstract:
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Engineering the interface between biomaterials and tissues is important to increase implant lifetime and avoid failures and revision surgeries. Permanent devices should enhance attachment and differentiation of stem cells, responsible for injured tissue repair, and simultaneously discourage bacterial colonization; this represents a major challenge. To take first steps towards such a multifunctional surface we propose merging topographical and biochemical cues on the surface of a clinically relevant material such as titanium. In detail, our strategy combines antibacterial nanotopographical features with integrin selective synthetic ligands that can rescue the adhesive capacity of the surfaces and instruct mesenchymal stem cell (MSC) response. To this end, a smooth substrate and two different high aspect ratio topographies have been produced and coated either with an avß3-selective peptidomimetic, an a5ß1-selective peptidomimetic, or an RGD/PHSRN peptidic molecule. Results showed that antibacterial effects of the substrates could be maintained when tested on pathogenic Pseudomonas aeruginosa. Further, functionalization increased MSC adhesion to the surfaces and the avß3-selective peptidomimetic-coated nanotopographies promoted osteogenesis. Such a dual physicochemical approach to achieve multifunctional surfaces represents a first step in the design of novel cell-instructive biomaterial surfaces. |
Abstract:
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Peer Reviewed |
Subject(s):
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-Àrees temàtiques de la UPC::Enginyeria dels materials -Surfaces -Superfícies |
Rights:
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Attribution 3.0 Spain
http://creativecommons.org/licenses/by/3.0/es/ |
Document type:
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Article - Published version Article |
Published by:
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Macmillan Publishers
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