Para acceder a los documentos con el texto completo, por favor, siga el siguiente enlace: http://hdl.handle.net/2445/114983

Glycerol is synthesized and secreted by adipocytes to dispose of excess glucose, via glycerogenesis and increased acyl-glycerol turnover
Rotondo, Floriana; Ho-Palma, Ana Cecilia; Remesar Betlloch, Xavier; Fernández López, José Antonio; Romero Romero, María del Mar; Alemany, Marià, 1946-
Universitat de Barcelona
White adipose tissue (WAT) produces large amounts of lactate and glycerol from glucose. We used mature epididymal adipocytes to analyse the relative importance of glycolytic versus lipogenic glycerol in adipocytes devoid of external stimuli. Cells were incubated (24/48 h) with 7/14 mM glucose; half of the wells contained 14C-glucose. We analysed glucose label fate, medium metabolites, and the expression of key genes coding for proteins controlling glycerol metabolism. The effects of initial glucose levels were small, but time of incubation increased cell activity and modified its metabolic focus. The massive efflux of lactate was uniform with time and unrelated to glucose concentration; however, glycerol-3P synthesis was higher in the second day of incubation, being largely incorporated into the glyceridesglycerol fraction. Glycerophosphatase expression was not affected by incubation. The stimulation of glycerogenic enzymes' expression was mirrored in lipases. The result was a shift from medium glycolytic to lipolytic glycerol released as a consequence of increased triacylglycerol turnover, in which most fatty acids were recycled. Production of glycerol seems to be an important primary function of adipocytes, maintained both by glycerogenesis and acyl-glycerol turnover. Production of 3C fragments may also contribute to convert excess glucose into smaller, more readily usable, 3C metabolites.
Teixit adipós
Glucosa
Glucòlisi
Adipose tissues
Glucose
Glycolysis
cc-by (c) Rotondo, Floriana et al., 2017
http://creativecommons.org/licenses/by/3.0/es
Artículo
info:eu-repo/semantics/publishedVersion
Nature Publishing Group
         

Mostrar el registro completo del ítem

Documentos relacionados

Otros documentos del mismo autor/a

Ho-Palma, Ana Cecilia; Rotondo, Floriana; Romero Romero, María del Mar; Memmolo, S.; Remesar Betlloch, Xavier; Fernández López, José Antonio; Alemany, Marià, 1946-
Rotondo, Floriana; Romero, María del Mar; Ho-Palma, Ana Cecilia; Remesar Betlloch, Xavier; Fernández López, José Antonio; Alemany, Marià, 1946-
Rotondo, Floriana; Sanz, Tania; Fernández López, José Antonio; Alemany, Marià, 1946-; Remesar Betlloch, Xavier
Romero Romero, María del Mar; Fernández López, José Antonio; Remesar Betlloch, Xavier; Alemany, Marià, 1946-
Sabater Martínez, David; Arriarán, Sofía; Romero Romero, María del Mar; Agnelli, Silvia; Fernández López, José Antonio; Remesar Betlloch, Xavier; Alemany, Marià, 1946-