Título:
|
Stabilization of telomeric i-motif structures by (2'S)-2'-deoxy-2'-C-methyl-cytidine residues
|
Autor/a:
|
Aviñó Andrés, Anna; Dellafiore, Maria; Gargallo Gómez, Raimundo; González, Carlos; Iribarren, Adolfo M.; Montserrat, Javier; Eritja i Casadellà, Ramon
|
Otros autores:
|
Universitat de Barcelona |
Abstract:
|
G-quadruplex and i-motif are tetraplex structures present in telomeres and promoter regions of oncogenes. The possibility of producing nanodevices with pH-sensitive functions has triggered the interest for modified oligonucleotides with improved structural properties. We synthesized C-rich oligonucleotides carrying conformationally restricted (2'S)-2'-deoxy-2'-C-methyl-cytidine units. The effect of this modified nucleoside on the stability of intramolecular i-motifs related to vertebrate telomere was investigated by means of spectroscopic methods (UV, CD and NMR). The replacement of selected positions of the C-core by the appropriate C-modified residues induces the formation of stable intercalated tetraplexes at pHs near neutrality. The study demonstrates the possibility of enhancing the stability of i-motif by chemical modifications. |
Materia(s):
|
-Àcids nucleics -Oligonucleòtids -G-estructures -Nucleic acids -Oligonucleotides -G-structures |
Derechos:
|
(c) Wiley-VCH, 2017
info:eu-repo/semantics/embargoedAccess |
Tipo de documento:
|
Artículo Artículo - Versión aceptada |
Editor:
|
Wiley-VCH
|
Compartir:
|
|