Title:
|
Treatment with G-CSF reduces acute myeloid leukemia blast viability in the presence of bone marrow stroma
|
Author:
|
Nomdedeu i Fàbrega, Meritxell; Lara Castillo, María Carmen; Etxabe, Amaia; Cornet Masana, Josep M.; Pratcorona, Marta; Díaz Beyá, Marina; Calvo, Xavier; Rozman, María; Costa, Dolors; Esteve Reyner, Jordi; Risueño, Ruth M.
|
Other authors:
|
Universitat de Barcelona |
Abstract:
|
BACKGROUND: The resulting clinical impact of the combined use of G-CSF with chemotherapy as a chemosensitizing strategy for treatment of acute myeloid leukemia (AML) patients is still controversial. In this study, the effect of ex vivo treatment with G-CSF on AML primary blasts was studied. METHODS: Peripheral blood mononuclear cells from AML patients were treated with G-CSF at increasing doses, alone or in co-culture with HS-5 stromal cells. Cell viability and surface phenotype was determined by flow cytometry 72 h after treatment. For clonogenicity assays, AML primary samples were treated for 18 h with G-CSF at increasing concentrations and cultured in methyl-cellulose for 14 days. Colonies were counted based on cellularity and morphology criteria. RESULTS: The presence of G-CSF reduced the overall viability of AML cells co-cultured with bone marrow stroma; whereas, in absence of stroma, a negligible effect was observed. Moreover, clonogenic capacity of AML cells was significantly reduced upon treatment with G-CSF. Interestingly, reduction in the AML clonogenic capacity correlated with the sensitivity to chemotherapy observed in vivo. CONCLUSIONS: These ex vivo results would provide a biological basis to data available from studies showing a clinical benefit with the use of G-CSF as a priming agent in patients with a chemosensitive AML and would support implementation of further studies exploring new strategies of chemotherapy priming in AML. |
Subject(s):
|
-Leucèmia mieloide -Hematologia -Medul·la òssia -Terapèutica -Factors de creixement -Myeloid leukemia -Hematology -Bone marrow -Therapeutics -Growth factors |
Rights:
|
cc-by (c) Nomdedeu i Fàbrega, Meritxell et al., 2015
http://creativecommons.org/licenses/by/3.0/es |
Document type:
|
Article Article - Published version |
Published by:
|
BioMed Central
|
Share:
|
|