To access the full text documents, please follow this link:

Development and characterization of novel microsatellites markers by next generation sequencing for the blue and red shrimp Aristeus antennatus.
Heras Mena, Sandra; Planella, Laia; Caldarazzo, Ilaria; Vera Rodríguez, Manuel; García Marín, José Luis; Roldán Borassi, María Inés
The blue and red shrimp, Aristeus antennatus, is a commercially important crustacean, in the Mediterranean Sea, which has been listed as a priority species for fishery management. Hypervariable microsatellite markers could be a useful tool to identify genetic stocks among geographically close fishing grounds. Potential microsatellite markers (97) identified from next-generation sequencing of an individual shrimp using a 454 GS Junior Pyrosequencer were tested on a preliminary panel of 15 individuals representing the four worldwide genetic stocks of the species from which 35 polymorphic loci were identified and used to characterize an additional 20 individuals from the Western Mediterranean Sea. In the Western Mediterranean sample, 32 out of 35 were polymorphic loci and the number of alleles per locus ranged from 2 to 14 and expected heterozygosity ranged from 0.050 to 0.968. No linkage disequilibrium was detected, indicating the independence of the loci. These novel microsatellites provide additional tools to address questions relating to genetic diversity, parentage studies and connectivity patterns of A. antennatus populations and help develop effective strategies to ensure long-term sustainability of this resource
Biologia de la conservació
Conservation biology
Biologia marina
Marine biology
Genètica de poblacions
Population genetics
Gambes -- Genètica -- Mediterrània, Mar
Shrimps -- Genetics -- Mediterranean Sea
Reconeixement 4.0 Espanya

Show full item record

Related documents

Other documents of the same author

Heras Mena, Sandra; Roldán Borassi, María Inés
Vera Rodríguez, Manuel; García Marín, José Luis; Martínez, Paulino; Araguas Solà, Rosa M.; Bouza da Costa, Carmen
Díez del Molino, David; Araguas Solà, Rosa M.; Vera Rodríguez, Manuel; Vidal Fàbrega, Oriol; Sanz Ball-llosera, Núria; García Marín, José Luis