Para acceder a los documentos con el texto completo, por favor, siga el siguiente enlace: http://hdl.handle.net/2117/78963

Decoupled illumination detection in light sheet microscopy for fast volumetric imaging
Olarte, Omar; Andilla, Jordi; Artigas García, David; Loza-Alvarez, Pablo
Universitat Politècnica de Catalunya. Institut de Ciències Fotòniques
Current microscopy demands the visualization of large three-dimensional samples with increased sensitivity, higher resolution, and faster speed. Several imaging techniques based on widefield, point-scanning, and light-sheet strategies have been designed to tackle some of these demands. Although successful, all these require the illuminated volumes to be tightly coupled with the detection optics to accomplish efficient optical sectioning. Here, we break this paradigm and produce optical sections from out-of-focus planes. This is done by extending the depth of field of the detection optics in a light-sheet microscope using wavefront-coding techniques. This passive technique allows accommodation of the light sheet at any place within the extended axial range. We show that this enables quick scanning of the light sheet across a volumetric sample. As a consequence, imaging speeds faster than twice the volumetric video rate (>70  volumes/s) can be achieved without needing to move the sample. These capabilities are demonstrated for volumetric imaging of fast dynamics in vivo as well as for fast, three-dimensional particle tracking.
Peer Reviewed
Àrees temàtiques de la UPC::Física
Imaging systems
imaging
Visió
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/publishedVersion
Artículo
OSA
         

Mostrar el registro completo del ítem

Documentos relacionados

Otros documentos del mismo autor/a

Aviles Espinosa, Rodrigo; Andilla, Jordi; Porcar-Guezenec, Rafael; Levecq, Xavier; Artigas García, David; Loza Álvarez, Pablo
Aviles Espinosa, Rodrigo; Andilla, Jordi; Porcar-Guezenec, Rafael; Olarte, Omar E.; Nieto, Marta; Levecq, Xavier; Artigas García, David; Loza Álvarez, Pablo
OLARTE, Omar Eduardo; Andilla, Jordi; Artigas García, David; Loza Álvarez, Pablo
Takayama, Osamu; Torner Sabata, Lluís; Artigas García, David
Psilodimitrakopoulos, Sotiris; Amat Roldán, Iván; Loza Álvarez, Pablo; Artigas García, David