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Imaging amylopectin's order in starch using 3-dimensional polarization SHG
Psilodimitrakopoulos, Sotiris; Amat Roldán, Iván; Artigas García, David; Loza Álvarez, Pablo
Universitat Politècnica de Catalunya. Departament de Teoria del Senyal i Comunicacions; Universitat Politècnica de Catalunya. FOTONICA - Grup de Recerca de Fotònica
In minimally destructive SHG biomedical imaging (high resolution optical slicing) is greatly desirable to extract the maximum of information from the light matter interaction. Here we develop a 3-D biophysical model and a methodology, which extracts molecular information below the experimental resolution limit. Firstly, it provides the pitch angle (SHG effective orientation) of the SHG source helix of the sample. This information is used to characterize and categorize the SHG sources among them. And secondly, it provides the degree of organization of the SHG source molecules. This can be used as a quantitative imaging biomarker able to characterize the degree of organization (homeostasis) of the sample. Here we applied the model in dried and hydrated wheat starch granules. Our results show that the SHG source molecule in starch is amylopectin. We also conclude that under hydration, the amylopectin molecules are further organized but they do not change structure. This organization is reflected to the width of the pitch angles pixels’ histograms’ distributions. The shorter the width is, the more organized the amylopectin molecules in starch are.
Peer Reviewed
Àrees temàtiques de la UPC::So, imatge i multimèdia::Creació multimèdia::Imatge digital
Àrees temàtiques de la UPC::Enginyeria de la telecomunicació
Image processing
Starch
Hydration
Polarization SHG imaging
Helical pitch angle
Amylopectin
Histograms’ width
Organization
Imatges -- Processament
info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
International Society for Photo-Optical Instrumentation Engineers (SPIE)
         

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