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A glucuronoxylan-specific xylanase from a new Paenibacillus favisporus strain isolated from tropical soil of Brazil
Padilha, I.Q.M.; Valenzuela Mayorga, Susana Valeria; Grisi, T.C.S.L.; Díaz Lucea, Pilar; de Araujo, D.A.M.; Pastor Blasco, Francisco I. Javier
Universitat de Barcelona
A new xylanolytic strain, Paenibacillus favisporus CC02-N2, was isolated from sugarcane plantation fi elds in Brazil. The strain had a xylan-degrading system with multiple enzymes, one of which, xylanase Xyn30A, was identifi ed and characterized. The enzyme is a single-domain xylanase belonging to family 30 of the glycosyl hydrolases (GH30). Xyn30A shows high activity on glucuronoxylans, with a Vmax of 267.2 U mg
1, a Km of 4.0 mg/ml, and a kcat of 13,333 min
1 on beechwood xylan, but it does not hydrolyze arabinoxylans. The three- dimensional structure of Xyn30A consists of a common (β/α)8 barrel linked to a side-chain-associated β-structure, similar to previously characterized GH30 xylanases. The hydrolysis products from glucuronoxylan were methylglucuronic-acid-substituted xylooligomers (acidic xylooligosaccharides). The enzyme bound to insoluble xylan but not to crystalline cellulose. Our results suggest a specifi c role for Xyn30A in xylan biodegradation in natural habitats. The enzyme is a good candidate for the production of tailored xylooligosaccharides for use in the food industry and in the biotechnological transformation of biomass. [Int Microbiol 2014; 17(3):175-184]
Bacils
Ecologia microbiana
Enzims microbians
Biopolímers
Sòls
Bacillus (Bacteria)
Microbial ecology
Microbial enzymes
Biopolymers
Soils
cc-by-nc-sa (c) Spanish Society for Microbiology (SEM) and Viguera Editores SL, 2014
http://creativecommons.org/licenses/by-nc-sa/3.0/es
Artículo
info:eu-repo/semantics/publishedVersion
Spanish Society for Microbiology (SEM) and Viguera Editores SL
         

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