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Cognate peptide-receptor ligand mapping by directed phage display
Stratmann, Thomas; Kang, Angray S.
Universitat de Barcelona
Background: A rapid phage display method for the elucidation of cognate peptide specific ligand for receptors is described. The approach may be readily integrated into the interface of genomic and proteomic studies to identify biologically relevant ligands. Methods: A gene fragment library from influenza coat protein haemagglutinin (HA) gene was constructed by treating HA cDNA with DNAse I to create 50 ¿ 100 bp fragments. These fragments were cloned into plasmid pORFES IV and in-frame inserts were selected. These in-frame fragment inserts were subsequently cloned into a filamentous phage display vector JC-M13-88 for surface display as fusions to a synthetic copy of gene VIII. Two well characterized antibodies, mAb 12CA5 and pAb 07431, directed against distinct known regions of HA were used to pan the library. Results: Two linear epitopes, HA peptide 112 ¿ 126 and 162¿173, recognized by mAb 12CA5 and pAb 07431, respectively, were identified as the cognate epitopes. Conclusion: This approach is a useful alternative to conventional methods such as screening of overlapping synthetic peptide libraries or gene fragment expression libraries when searching for precise peptide protein interactions, and may be applied to functional proteomics.
Pèptids
Proteòmica
Synthetic peptide libraries
Gene fragment expression libraries
cc-by, (c) Stratmann et al., 2005
http://creativecommons.org/licenses/by/2.0/
Artículo
BioMed Central
         

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Saavedra Ávila, Noemí Alejandra; SenGupta, Upasana; Sánchez, Begoña; Sala, Ester; Haba, Laura; Stratmann, Thomas; Verdaguer Autonell, Joan; Mauricio Puente, Dídac; Mezquita, Bélen; Ropero, Ana Belén; Nadal, Ángel; Mora Giral, Concepció
Pujol Autonell, Irma; Serracant Prat, Arnau; Cano Sarabia, Antonia María; Ampudia, Rosa M.; Rodríguez Fernández, Silvia; Sánchez (Sànchez Pla), Álex; Izquierdo, Cristina; Stratmann, Thomas; Puig Domingo, Manuel; Maspoch, Daniel; Verdaguer Autonell, Joan; Vives Pi, Marta
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