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miR-143 Interferes with ERK5 Signaling, and Abrogates Prostate Cancer Progression in Mice
Clapé, Cyrielle; Fritz, Vanessa; Henriquet, Corinne; Apparailly, Florence; Fernández Ruiz, Pedro Luis; Iborra, François; Avancès, Christophe; Villalba, Martin; Culine, Stéphane; Fajas, Lluis
Universitat de Barcelona
Abstract Background: Micro RNAs are small, non-coding, single-stranded RNAs that negatively regulate gene expression at the post-transcriptional level. Since miR-143 was found to be down-regulated in prostate cancer cells, we wanted to analyze its expression in human prostate cancer, and test the ability of miR-43 to arrest prostate cancer cell growth in vitro and in vivo. Results: Expression of miR-143 was analyzed in human prostate cancers by quantitative PCR, and by in situ hybridization. miR-143 was introduced in cancer cells in vivo by electroporation. Bioinformatics analysis and luciferase-based assays were used to determine miR-143 targets. We show in this study that miR-143 levels are inversely correlated with advanced stages of prostate cancer. Rescue of miR-143 expression in cancer cells results in the arrest of cell proliferation and the abrogation of tumor growth in mice. Furthermore, we show that the effects of miR-143 are mediated, at least in part by the inhibition of extracellular signal-regulated kinase-5 (ERK5) activity. We show here that ERK5 is a miR-143 target in prostate cancer. Conclusions: miR-143 is as a new target for prostate cancer treatment.
Micro RNAs
Càncer de pròstata
Bioinformàtica
MicroRNAs
Prostate cancer
Bioinformatics
cc-by (c) Clapé, C. et al., 2009
http://creativecommons.org/licenses/by/3.0/es
Article
info:eu-repo/semantics/publishedVersion
Public Library of Science (PLoS)
         

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