dc.contributor |
Universitat de Barcelona |
dc.contributor.author |
Astefanei, Alina |
dc.contributor.author |
Núñez Burcio, Oscar |
dc.contributor.author |
Galcerán Huguet, M. Teresa |
dc.date |
2016-05-30T12:34:12Z |
dc.date |
2016-05-30T12:34:12Z |
dc.date |
2014-09-02 |
dc.date |
2016-05-30T12:34:17Z |
dc.identifier.citation |
0021-9673 |
dc.identifier.citation |
642832 |
dc.identifier.uri |
http://hdl.handle.net/2445/99043 |
dc.format |
11 p. |
dc.format |
application/pdf |
dc.language.iso |
eng |
dc.publisher |
Elsevier B.V. |
dc.relation |
Versió postprint del document publicat a: http://dx.doi.org/10.1016/j.chroma.2014.08.089 |
dc.relation |
Journal of Chromatography A, 2014, vol. 1365, p. 61-71 |
dc.relation |
http://dx.doi.org/10.1016/j.chroma.2014.08.089 |
dc.rights |
(c) Elsevier B.V., 2014 |
dc.rights |
info:eu-repo/semantics/openAccess |
dc.subject |
Ful·lerens |
dc.subject |
Espectrometria de masses |
dc.subject |
Cromatografia de líquids d'alta resolució |
dc.subject |
Mostreig mediambiental |
dc.subject |
Fullerenes |
dc.subject |
Mass spectrometry |
dc.subject |
High performance liquid chromatography |
dc.subject |
Environmental sampling |
dc.title |
Analysis of C60-fullerene fullerene derivatives and pristine fullerenes in environmental samples by ultrahigh performance liquid chromatography-atmospheric pressure photoionization-mass spectrometry |
dc.type |
info:eu-repo/semantics/article |
dc.type |
info:eu-repo/semantics/acceptedVersion |
dc.description.abstract |
In this work, a method is proposed for the simultaneous analysis of several pristine fullerenes (C60, C70, C76, C78, and C84) and three C60-fullerene derivatives (N-methyl fulleropyrrolidine, [6,6]-Phenyl C61 butyric acid methyl ester and [6,6]-Phenyl C61 butyric acid buthyl ester) in environmental samples. The method involves the use of ultrahigh performance liquid chromatography coupled to atmospheric pressure photoionization mass spectrometry (UHPLC-APPI-MS) and allowed the chromatographic separation in less than 4.5 minutes. The product ions from tandem mass spectrometry studies of fullerene derivatives, were characterized and the most abundant one (m/z 720), corresponding to [C60]-¿, was selected for quantitation. Selected reaction monitoring (SRM at 0.7 m/z FWHM) by acquiring two transitions using both isotopic cluster ions [M]-¿ and [M+1]-¿ as precursor ions was proposed for quantitation and confirmation purposes. For pristine fullerenes, highly-selective selected ion monitoring (H-SIM) acquisition mode by monitoring the isotopic cluster ions [M]-¿ and [M+1]-¿ was used. Pressurized solvent extraction conditions were optimized in order to improve recoveries of the studied fullerene compounds from sediment samples. Values up to 87-92% for C60-fullerene derivatives and lower but still acceptable, 70-80%, for pristine fullerenes were obtained. Method limits of quantitation (MLOQs) ranging from 1.5 pg L-1to 5.5 ng L-1 in water samples and from 0.1 ng Kg-1 to 523 ng Kg-1 in sediments were obtained with good precision (relative standard deviations always lower than 13%). The applicability of the developed method was evaluated by analyzing several environmental samples such as sediments and pond water and the detected levels for C60-fullerene derivatives were of 0.1-2.7 ng Kg-1and 1.5-8.5 pg L-1, respectively. C60 and C70 were the only pristine fullerenes detected in the analyzed samples (0.1-7.2 ng Kg-1 in sediments and 9-330 pg L-1 in water pond samples). |