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Using Massive Parallel Sequencing for the Development, Validation, and Application of Population Genetics Markers in the Invasive Bivalve Zebra Mussel (Dreissena polymorpha)
Peñarrubia Lozano, Luis; Sanz Ball-llosera, Núria; Pla Zanuy, Carles; Vidal Fàbrega, Oriol; Viñas de Puig, Jordi
The zebra mussel (Dreissena polymorpha, Pallas, 1771) is one of the most invasive speciesof freshwater bivalves, due to a combination of biological and anthropogenic factors. Oncethis species has been introduced to a new area, individuals form dense aggregations thatare very difficult to remove, leading to many adverse socioeconomic and ecological consequences.In this study, we identified, tested, and validated a new set of polymorphic microsatelliteloci (also known as SSRs, Single Sequence Repeats) using a Massive ParallelSequencing (MPS) platform. After several pruning steps, 93 SSRs could potentially be amplified.Out of these SSRs, 14 were polymorphic, producing a polymorphic yield of 15.05%.These 14 polymorphic microsatellites were fully validated in a first approximation of the geneticpopulation structure of D. polymorpha in the Iberian Peninsula. Based on this polymorphicyield, we propose a criterion for establishing the number of SSRs that requirevalidation in similar species, depending on the final use of the markers. These results couldbe used to optimize MPS approaches in the development of microsatellites as geneticmarkers, which would reduce the cost of this process
Genètica de poblacions
Population genetics
Musclos d’aigua dolça -- Genètica
Freshwater mussels -- Genetics
Animals invasors
Invasions biològiques
Introduced organisms
Biological invasions
Attribution 3.0 Spain
http://creativecommons.org/licenses/by/3.0/es/
Artículo
info:eu-repo/semantics/publishedVersion
Public Library of Science (PLoS)
         

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