Title:
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Ultra rapid in vivo screening for anti-Alzheimer anti-amyloid drugs
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Author:
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Espargaró Colomé, Alba; Medina, Aina; Di Pietro, Ornella; Muñoz-Torrero López-Ibarra, Diego; Sabaté Lagunas, Raimon
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Other authors:
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Universitat de Barcelona |
Abstract:
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More than 46 million people worldwide suffer from Alzheimer's disease. A large number of potential treatments have been proposed; among these, the inhibition of the aggregation of amyloid β-peptide (Aβ), considered one of the main culprits in Alzheimer's disease. Limitations in monitoring the aggregation of Aβ in cells and tissues restrict the screening of anti-amyloid drugs to in vitro studies in most cases. We have developed a simple but powerful method to track Aβ aggregation in vivo in realtime, using bacteria as in vivo amyloid reservoir. We use the specific amyloid dye Thioflavin-S (Th-S) to stain bacterial inclusion bodies (IBs), in this case mainly formed of Aβ in amyloid conformation. Th-S binding to amyloids leads to an increment of fluorescence that can be monitored. The quantification of the Th-S fluorescence along the time allows tracking Aβ aggregation and the effect of potential antiaggregating agents. |
Subject(s):
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-Malaltia d'Alzheimer -Pèptids -Agregació (Química) -Disseny de medicaments -Alzheimer's disease -Peptides -Aggregation (Chemistry) -Drug design |
Rights:
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cc-by-nc-nd (c) Espargaró Colomé, Alba et al., 2016
http://creativecommons.org/licenses/by-nc-nd/3.0/es |
Document type:
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Article Article - Published version |
Published by:
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Nature Publishing Group
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