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Tuning Mesenchymal Stem Cell Response onto Titanium-Niobium-Hafnium Alloy by Recombinant Fibronectin Fragments
Herranz-Diez, Carolina; Mas Moruno, Carlos; Neubauer, S; Kessler, H; Gil Mur, Javier; Pegueroles Neyra, Marta; Manero Planella, José María; Guillem Martí, Jordi
Universitat Politècnica de Catalunya. Departament de Ciència dels Materials i Enginyeria Metal·lúrgica; Universitat Politècnica de Catalunya. BIBITE - Biomaterials, Biomecànica i Enginyeria de Teixits
Since metallic biomaterials used for bone replacement possess low bioactivity, the use of cell adhesive moieties is a common strategy to improve cellular response onto these surfaces. In recent years, the use of recombinant proteins has emerged as an alternative to native proteins and short peptides owing to the fact that they retain the biological potency of native proteins, while improving their stability. In the present study, we investigated the biological effect of two different recombinant fragments of fibronectin, spanning the 8–10th and 12–14th type III repeats, covalently attached to a new TiNbHf alloy using APTES silanization. The fragments were studied separately and mixed at different concentrations and compared to a linear RGD, a cyclic RGD and the full-length fibronectin protein. Cell culture studies using rat mesenchymal stem cells demonstrated that low to medium concentrations (30% and 50%) of type III 8–10th fragment mixed with type III 12–14th fragment stimulated cell spreading and proliferation compared to RGD peptides and the fragments separately. On the other hand, type III 12–14th fragment alone or mixed at low volume percentages =50% with type III 8–10th fragment increased alkaline phosphatase levels compared to the other molecules. These results are significant for the understanding of the role of fibronectin recombinant fragments in cell responses and thus to design bioactive coatings for biomedical application
Peer Reviewed
Àrees temàtiques de la UPC::Enginyeria dels materials
Titanium alloys
Biomedical materials
recombinant protein
cell adhesive peptides
mesenchymal stem cells
titanium alloy
Titani -- Aliatges
Materials biomèdics

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