Para acceder a los documentos con el texto completo, por favor, siga el siguiente enlace: http://hdl.handle.net/2445/97209

Determination of Methotrexate in pH-Sensitive Chitosan Nanoparticles by Validated RP-LC and UV Spectrophotometric Methods
Nogueira D.R.; Macedo, Letícia B.; Scheeren, Laís E.; Mitjans Arnal, Montserrat; Infante Martínez-Pardo, Ma. Rosa; Rolim, Clarice M.B.; Vinardell Martínez-Hidalgo, Ma. Pilar
Universitat de Barcelona
Nanotechnology-based drug delivery systems are in constant development and, therefore, it is of great importance to have rapid, efficient and accurate analytical methodology to quantify the encapsulated drugs. Here, simple and fast methods, by reversed-phase liquid chromatography (RP-LC) and UV spectrophotometry, were developed and validated for the determination of methotrexate (MTX) in pH-sensitive chitosan nanoparticles (CS-NPs). NPs were prepared using a modified ionotropic complexation process, in which was included a surfactant derived from Nα,Nε-dioctanoyl lysine with an inorganic sodium counterion. The RP-LC method was carried out on a Waters XBridgeTM C18 column (250 mm x 4.6 mm I.D., 5μm), with mobile phase consisted of potassium phosphate buffer (0.05 M, pH 3.2): acetonitrile (86:14, v/v), and UV detection set at 303 nm. The analyses of MTX content by the UV method were also accomplished at 303 nm, using 0.1 M sodium hydroxide as diluent. The measurements were linearly correlated with concentration for both methods in the 1 - 30 μg/mL range (r > 0.9999). The specificity tests showed that there was no interference of the NP components on the quantitative analyses. Precision (repeatability and intermediate precision) was demonstrated by a relative standard deviation lower than 1.5%, whereas the accuracy was assessed by the recovery of MTX from sample matrices, given mean value of ~99%. The proposed methods were applied for the analyses of MTX in different batches of NPs, and the results showed non-significant differences (p > 0.05) between the values obtained with both methodologies. Moreover, the RP-LC method was successfully used to determine the drug entrapment efficiency, and to quantify MTX during in vitro release assays and photolytic degradation studies. In conclusion, the validated methods are suitable to assay MTX in pH-sensitive CS-NPs without any interference from the polymer or surfactant.
Quitosan
Nanopartícules
Medicaments antineoplàstics
Metotrexat
Agents tensioactius
Cromatografia
Espectroscòpia
Chitosan
Nanoparticles
Antineoplastic agents
Methotrexate
Surface active agents
Chromatography
Spectrum analysis
cc-by-nc (c) Nogueira D.R. et al., 2014
http://creativecommons.org/licenses/by-nc/3.0/es
Artículo
info:eu-repo/semantics/publishedVersion
Pharma Publisher
         

Mostrar el registro completo del ítem

Documentos relacionados

Otros documentos del mismo autor/a

Scheeren, Laís E.; Nogueira, Daniele R.; Macedo, Letícia B.; Vinardell Martínez-Hidalgo, Ma. Pilar; Mitjans Arnal, Montserrat; Infante Martínez-Pardo, Ma. Rosa; Rolim, Clarice M.B.
Nogueira, Daniele R.; Scheeren, Laís E.; Macedo, Letícia B.; Marcolino, Ana Isa P.; Mitjans Arnal, Montserrat; Vinardell Martínez-Hidalgo, Ma. Pilar; Infante Martínez-Pardo, Ma. Rosa; Farooqi, Ammad A.; Rolim, Clarice M.B.
Nogueira, Daniele R.; Scheeren, Laís E.; Vinardell Martínez-Hidalgo, Ma. Pilar; Mitjans Arnal, Montserrat; Infante Martínez-Pardo, Ma. Rosa; Rolim, Clarice M.B.
Nogueira, Daniele R.; Mitjans Arnal, Montserrat; Rolim, Clarice M.B.; Vinardell Martínez-Hidalgo, Ma. Pilar
Colomer Flores, Aurora; Pinazo Gassol, Aurora; Garcia Ramón, Maria Teresa; Mitjans Arnal, Montserrat; Vinardell Martínez-Hidalgo, Ma. Pilar; Infante Martínez-Pardo, Ma. Rosa; Martínez Ocaña, Verónica; Pérez Muñoz, Lourdes