The objective of the thesis is to illustrate the multiple applications of ATPS in the biotechnological industry and to learn the methodologies applied for the partition of biomacromolecules using these systems. To achieve these objectives several experiments have been performed to find the most appropriate aqueous two-phase system to partition the chosen molecules. A methodology has been designed and applied to characterize and perform the separation with the ATPS. The results extracted followed the expected values validating the methodology imposed throughout experimentation. The conclusions are that this is, indeed, a highly appropriate separation option for the tested macromolecules and that the future of biotechnology industry could be highly bound to this mild separation methods.