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Prostate tumor OVerexpressed-1 (PTOV1) down-regulates HES1 and HEY1 notch targets genes and promotes prostate cancer progression
Alaña, Lide; Sesé, M.; Cánovas, Verónica; Punyal, Yolanda; Fernández, Yolanda; Abasolo, Ibane; Torres, Inés de; Ruiz, Cristina; Espinosa Blay, Lluís; Bigas Salvans, Anna; Ramón y Cajal Agüeras, Santiago; Fernández Ruiz, Pedro Luis; Serras Rigalt, Florenci; Corominas, Montserrat (Corominas Guiu); Thomson, T.M.; Paciucci, R.
Universitat de Barcelona
Background PTOV1 is an adaptor protein with functions in diverse processes, including gene transcription and protein translation, whose overexpression is associated with a higher proliferation index and tumor grade in prostate cancer (PC) and other neoplasms. Here we report its interaction with the Notch pathway and its involvement in PC progression. Methods Stable PTOV1 knockdown or overexpression were performed by lentiviral transduction. Protein interactions were analyzed by co-immunoprecipitation, pull-down and/or immunofluorescence. Endogenous gene expression was analyzed by real time RT-PCR and/or Western blotting. Exogenous promoter activities were studied by luciferase assays. Gene promoter interactions were analyzed by chromatin immunoprecipitation assays (ChIP). In vivo studies were performed in the Drosophila melanogaster wing, the SCID-Beige mouse model, and human prostate cancer tissues and metastasis. The Excel package was used for statistical analysis [...].
Càncer de pròstata
Marcadors tumorals
Prostate cancer
Tumor markers
cc-by (c) Alaña, Lide et al., 2014
http://creativecommons.org/licenses/by/3.0/es
Article
info:eu-repo/semantics/publishedVersion
BioMed Central
         

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