Title:
|
Effect of Theobroma cacao flavonoids on immune activation of a lymphoid cell line
|
Author:
|
Ramiro Puig, Emma; Franch i Masferrer, Àngels; Castellote i Bargalló, M. Cristina; Andrés Lacueva, Ma. Cristina; Izquierdo Pulido, María Luz; Castell, Margarida
|
Other authors:
|
Universitat de Barcelona |
Abstract:
|
We analysed the effect of (-)-epicatechin and cocoa extract on the activation of a lymphoid cell line. Particularly the expression of IL-2 receptor alpha (IL-2Ralpha or CD25) and, the secretion of IL-2 and IL-4 were established after flavonoid treatment. Two media culture conditions (1 and 10 % of fetal calf serum supplementation) and the different moments of flavonoid addition (simultaneously or 2 h before cell-activation) were compared. IL-2Ralpha (CD25) expression on activated cells was significantly reduced by epicatechin and cocoa extract in a dose-dependent manner, achieving the highest inhibition of about 50 % when flavonoids were added 2 h before stimulation. IL-2 secretion was also inhibited by the presence of both epicatechin and cocoa extract, displaying 60 and 75 % of inhibition, respectively. Cocoa flavonoids were also able to enhance 3-4.5-fold IL-4 release. In summary, cocoa extract down-modulated T lymphocyte activation and therefore the acquired immune response. This fact could be important in some states of the immune system hyperactivity such as autoimmune or chronic inflammatory diseases. |
Subject(s):
|
-Cacau -Flavonoides -Immunologia -Cocoa -Flavonoids -Immunology |
Rights:
|
(c) Cambridge University Press, 2005
|
Document type:
|
Article Article - Published version |
Published by:
|
Cambridge University Press
|
Share:
|
|