To access the full text documents, please follow this link: http://hdl.handle.net/2445/57313

Long-Term Increased Carnitine Palmitoyltransferase 1A Expression in Ventromedial Hypotalamus Causes Hyperphagia and Alters the Hypothalamic Lipidomic Profile
Mera Nanín, Paula; Mir, Joan Francesc; Fabriàs Domingo, Gemma; Casas Brugulat, Josefina; Costa, Ana S.H.; Malandrino, Maria Ida; Fernández López, José Antonio; Remesar Betlloch, Xavier; Gao, Su; Chohnan, Shigeru; Rodríguez-Peña, Maria Sol; Petry, Harald; Asins Muñoz, Guillermina; García Hegardt, Fausto; Herrero Rodríguez, Laura; Serra i Cucurull, Dolors
Universitat de Barcelona
Lipid metabolism in the ventromedial hypothalamus (VMH) has emerged as a crucial pathway in the regulation of feeding and energy homeostasis. Carnitine palmitoyltransferase (CPT) 1A is the rate-limiting enzyme in mitochondrial fatty acid boxidation and it has been proposed as a crucial mediator of fasting and ghrelin orexigenic signalling. However, the relationship between changes in CPT1A activity and the intracellular downstream effectors in the VMH that contribute to appetite modulation is not fully understood. To this end, we examined the effect of long-term expression of a permanently activated CPT1A isoform by using an adeno-associated viral vector injected into the VMH of rats. Peripherally, this procedure provoked hyperghrelinemia and hyperphagia, which led to overweight, hyperglycemia and insulin resistance. In the mediobasal hypothalamus (MBH), long-term CPT1AM expression in the VMH did not modify acyl-CoA or malonyl-CoA levels. However, it altered the MBH lipidomic profile since ceramides and sphingolipids increased and phospholipids decreased. Furthermore, we detected increased vesicular c-aminobutyric acid transporter (VGAT) and reduced vesicular glutamate transporter 2 (VGLUT2) expressions, both transporters involved in this orexigenic signal. Taken together, these observations indicate that CPT1A contributes to the regulation of feeding by modulating the expression of neurotransmitter transporters and lipid components that influence the orexigenic pathways in VMH.
Carnitina palmitoïl-transferasa 1
Esfingolípids
Glucosa
Hipotàlem
Hormones peptídiques
Metabolisme dels glúcids
Metabolisme dels lípids
Rates (Animals de laboratori)
Teixit adipós
Carnitine palmitoyltransferase I
Sphingolipids
Glucose
Hypothalamus
Peptide hormones
Carbohydrate metabolism
Lipid metabolism
Rats as laboratory animals
Adipose tissues
cc-by (c) Mera Nanín, Paula et al., 2014
http://creativecommons.org/licenses/by/3.0/es
Article
info:eu-repo/semantics/publishedVersion
Public Library of Science (PLoS)
         

Show full item record

Related documents

Other documents of the same author

Mera Nanín, Paula; Mir, Joan Francesc; Fabriàs Domingo, Gemma; Casas Brugulat, Josefina; Costa, Ana S.H.; Malandrino, Maria Ida; Fernández López, José Antonio; Remesar Betlloch, Xavier; Gao, Su; Chohnan, Shigeru; Rodríguez-Peña, Maria Sol; Petry, Harald; Asins Muñoz, Guillermina; García Hegardt, Fausto; Herrero Rodríguez, Laura; Serra i Cucurull, Dolors
Mera Nanín, Paula; Mir, Joan Francesc; Fabriàs Domingo, Gemma; Casas Brugulat, Josefina; Costa, Ana S.H.; Malandrino, Maria Ida; Fernández López, José Antonio; Remesar Betlloch, Xavier; Gao, Su; Chohnan, Shigeru; Rodríguez-Peña, Maria Sol; Petry, Harald; Asins Muñoz, Guillermina; García Hegardt, Fausto; Herrero Rodríguez, Laura; Serra i Cucurull, Dolors
Herrero Rodríguez, Laura; Mera Nanín, Paula; Orellana Gavaldà, Josep Maria; Gurunathan, Chandrashekaran; Malandrino, Maria Ida; Mir, Joan Francesc; Makowski, Kamil; Pozo, Macarena; Arasa, Maria del Mar; Ariza Piquer, Xavier; Asins Muñoz, Guillermina; García Gómez, Jordi; García Hegardt, Fausto; Serra i Cucurull, Dolors
Serra i Cucurull, Dolors; Mera Nanín, Paula; Malandrino, Maria Ida; Mir, Joan Francesc; Herrero Rodríguez, Laura
Serra i Cucurull, Dolors; Mera Nanín, Paula; Malandrino, Maria Ida; Mir, Joan Francesc; Herrero Rodríguez, Laura
 

Coordination

 

Supporters