Para acceder a los documentos con el texto completo, por favor, siga el siguiente enlace: http://hdl.handle.net/2445/66250

Induction of prophages by fluoroquinolones in streptococcus pneumoniae: implications for emergence of resistance in genetically-related clones
López, Elena; Domènech, Arnau; Ferrándiz, María José; Frias, Maria Joao; Ardanuy Tisaire, María Carmen; Ramirez, Mario; García, Ernesto; Liñares Louzao, Josefina; De la Campa, Adela G.
Antibiotic resistance in Streptococcus pneumoniae has increased worldwide by the spread of a few clones. Fluoroquinolone resistance occurs mainly by alteration of their intracellular targets, the type II DNA topoisomerases, which is acquired either by point mutation or by recombination. Increase in fluoroquinolone-resistance may depend on the balance between antibiotic consumption and the cost that resistance imposes to bacterial fitness. In addition, pneumococcal prophages could play an important role. Prophage induction by fluoroquinolones was confirmed in 4 clinical isolates by using Southern blot hybridization. Clinical isolates (105 fluoroquinolone-resistant and 160 fluoroquinolone-susceptible) were tested for lysogeny by using a PCR assay and functional prophage carriage was studied by mitomycin C induction. Fluoroquinolone-resistant strains harbored fewer inducible prophages (17/43) than fluoroquinolone-susceptible strains (49/70) (P = 0.0018). In addition, isolates of clones associated with fluoroquinolone resistance [CC156 (3/25); CC63 (2/20), and CC81 (1/19)], had lower frequency of functional prophages than isolates of clones with low incidence of fluoroquinolone resistance [CC30 (4/21), CC230 (5/20), CC62 (9/21), and CC180 (21/30)]. Likewise, persistent strains from patients with chronic respiratory diseases subjected to fluoroquinolone treatment had a low frequency of inducible prophages (1/11). Development of ciprofloxacin resistance was tested with two isogenic strains, one lysogenic and the other non-lysogenic: emergence of resistance was only observed in the non-lysogenic strain. These results are compatible with the lysis of lysogenic isolates receiving fluoroquinolones before the development of resistance and explain the inverse relation between presence of inducible prophages and fluoroquinolone-resistance.
Pneumococs
Estreptococs
Resistència als medicaments
Malalties de l'aparell respiratori
Malalties cròniques
Streptococcus pneumonia
Streptococcus
Drug resistance
Respiratory diseases
Chronic diseases
cc-by (c) López, Elena et al., 2014
http://creativecommons.org/licenses/by/3.0/es
Artículo
info:eu-repo/semantics/publishedVersion
Public Library of Science (PLoS)
         

Mostrar el registro completo del ítem

Documentos relacionados

Otros documentos del mismo autor/a

López, Elena; Domènech, Arnau; Ferrándiz, María José; Frias, Maria Joao; Ardanuy Tisaire, María Carmen; Ramirez, Mario; García, Ernesto; Liñares Louzao, Josefina; De la Campa, Adela G.
Domènech, Arnau; Ardanuy Tisaire, María Carmen; Pallarés Giner, Roman; Grau, Immaculada; Santos Pérez, Salud; De la Campa, Adela G.; Liñares Louzao, Josefina
Domènech, Arnau; Ardanuy Tisaire, María Carmen; Pallarés Giner, Roman; Grau, Immaculada; Santos Pérez, Salud; De la Campa, Adela G.; Liñares Louzao, Josefina
Ardanuy Tisaire, María Carmen; De la Campa, Adela G.; García, Ernesto; Fenoll, Asunción; Calatayud, Laura; Cercenado, Emilia; Pérez Trallero, Emilio; Bouza, Emilio; Liñares Louzao, Josefina
Ardanuy Tisaire, María Carmen; De la Campa, Adela G.; García, Ernesto; Fenoll, Asunción; Calatayud, Laura; Cercenado, Emilia; Pérez Trallero, Emilio; Bouza, Emilio; Liñares Louzao, Josefina