Para acceder a los documentos con el texto completo, por favor, siga el siguiente enlace: http://hdl.handle.net/2445/66126

Characterization of nontypable haemophilus influenzae isolates recovered from adult patients with underlying chronic lung disease reveals genotypic and phenotypic traits associated with persistent infection
Garmendia, Junkal; Viadas, Cristina; Calatayud, Laura; Mell, Joshua Chang; Martí Lliteras, Pau; Euba, Begoña; Llobet, Enrique; Gil, Carmen; Bengoechea, José Antonio; Redfield, Rosemary J.; Liñares Louzao, Josefina
Nontypable Haemophilus influenzae (NTHi) has emerged as an important opportunistic pathogen causing infection in adults suffering obstructive lung diseases. Existing evidence associates chronic infection by NTHi to the progression of the chronic respiratory disease, but specific features of NTHi associated with persistence have not been comprehensively addressed. To provide clues about adaptive strategies adopted by NTHi during persistent infection, we compared sequential persistent isolates with newly acquired isolates in sputa from six patients with chronic obstructive lung disease. Pulse field gel electrophoresis (PFGE) identified three patients with consecutive persistent strains and three with new strains. Phenotypic characterisation included infection of respiratory epithelial cells, bacterial self-aggregation, biofilm formation and resistance to antimicrobial peptides (AMP). Persistent isolates differed from new strains in showing low epithelial adhesion and inability to form biofilms when grown under continuous-flow culture conditions in microfermenters. Self-aggregation clustered the strains by patient, not by persistence. Increasing resistance to AMPs was observed for each series of persistent isolates; this was not associated with lipooligosaccharide decoration with phosphorylcholine or with lipid A acylation. Variation was further analyzed for the series of three persistent isolates recovered from patient 1. These isolates displayed comparable growth rate, natural transformation frequency and murine pulmonary infection. Genome sequencing of these three isolates revealed sequential acquisition of single-nucleotide variants in the AMP permease sapC, the heme acquisition systems hgpB, hgpC, hup and hxuC, the 3-deoxy-D-manno-octulosonic acid kinase kdkA, the long-chain fatty acid transporter ompP1, and the phosphoribosylamine glycine ligase purD. Collectively, we frame a range of pathogenic traits and a repertoire of genetic variants in the context of persistent infection by NTHi.
Trobareu correccions del document a: http://dx.doi.org/10.1371/journal.pone.0107686
Malalties pulmonars obstructives cròniques
Malalties dels pulmons
Bacteris patògens
Infeccions oportunistes
Fenotip
Chronic obstructive pulmonary diseases
Pulmonary diseases
Pathogenic bacteria
Opportunistic infections
Phenotype
cc-by (c) Garmendia, Junkal et al., 2014
http://creativecommons.org/licenses/by/3.0/es
Artículo
info:eu-repo/semantics/publishedVersion
Public Library of Science (PLoS)
         

Mostrar el registro completo del ítem

Documentos relacionados

Otros documentos del mismo autor/a

Garmendia, Junkal; Viadas, Cristina; Calatayud, Laura; Mell, Joshua Chang; Martí Lliteras, Pau; Euba, Begoña; Llobet, Enrique; Gil, Carmen; Bengoechea, José Antonio; Redfield, Rosemary J.; Liñares Louzao, Josefina
Martí Lliteras, Pau; López Gómez, A.; Mauro, S.; Hood, D.W.; Viadas, Cristina; Calatayud, Laura; Morey, P.; Servin, A.; Liñares Louzao, Josefina; Oliver, A.; Bengoechea, José Antonio; Garmendia, Junkal
Martí Lliteras, Pau; López Gómez, A.; Mauro, S.; Hood, D.W.; Viadas, Cristina; Calatayud, Laura; Morey, P.; Servin, A.; Liñares Louzao, Josefina; Oliver, A.; Bengoechea, José Antonio; Garmendia, Junkal
March, C.; Cano, V.; Moranta, D.; Llobet, Enrique; Pérez-Gutiérrez, C.; Tomàs Magaña, Juan; Suarez, T.; Garmendia, Junkal; Bengoechea, José Antonio
Puig Pitarch, Carmen; Grau, Immaculada; Martí Martí, Sara; Tubau, Fe; Calatayud, Laura; Pallarés Giner, Roman; Liñares Louzao, Josefina; Ardanuy Tisaire, María Carmen