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Increasing yield of nanocrystalline cellulose preparation process by a cellulase pretreatment
Beltramino Heffes, Facundo; Roncero Vivero, María Blanca; Vidal Lluciá, Teresa; Torres López, Antonio Luis; Valls Vidal, Cristina
Universitat Politècnica de Catalunya. Departament d'Enginyeria Tèxtil i Paperera; Universitat Politècnica de Catalunya. CELBIOTECH - Grup de Recerca: Enginyeria Paperera
In this work the introduction of a cellulase treatment prior to NCC isolation was assessed. NCC was produced using sulfuric acid at two different concentrations (62 and 64% wt.). The effect of pore size for filtration step was also assessed. The smaller acid dose leaded to yields up to 65–70% and average size up to 160 nm. It also produced crystals with reduced sulfur content (0.6–1%). Cellulase pretreatment influenced NCC characteristics, as it increased overall yield a 12%, increased average particle size around 35 nm and reduced NCC sulfur content up to a 0.8%. We found that different conditions of enzymatic treatments led to quantitative differences on their effects on NCC. Acetate buffer used for enzymatic treatments was found to counteract effects of acid. The evidence presented in this work suggested that pretreating fibers with this cellulase represents a very interesting option to partially replace chemicals on NCC isolation
Àrees temàtiques de la UPC::Enginyeria paperera::Primeres matèries papereres::Cel·lulosa
Cellulose--Chemistry
Cellulase
Nanocrystalline cellulose
Cellulase
Enzymatic treatment
Yield increase
Acid hydrolysis
Cel·lulosa microcristal·lina
Cel·lulasa
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