A new mathematical model to solve the Mixed-Model Sequencing Problem with Work overload minimization is formulated. The model incorporates productive, social and legal aspects in order to move the theory problem closer to the actual industrial environments. Specifically, there are considered the variation of work pace of workers throughout the workday to increase the completed work; the conditions of occupancy level of workers imposed by the collective agreements; and the idea of keeping constant the production mix through the sequence leading both to a balance between the required workloads at stations and regular consumption of components. Indeed, by means of a case study linked to Nissan, a gain of over 98% is achieved in terms of regular cumulative production and required work, while performing the 100% of required work and following legal restrictions of operators’ saturation.