Título:
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Profiling of oxygen in biofilms using individually addressable disk microelectrodes on a microfabricated needle
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Autor/a:
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Moya Lara, Ana; Guimerà Villalba, Xavier; Del Campo, Francisco Javier; Prats-Alfonso, Elisabet; Dorado Castaño, Antonio David; Baeza Labat, Mireia; Villa, Rosa; Gabriel Buguña, David; Gamisans Noguera, Javier; Gabriel Buguña, Gemma
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Otros autores:
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Universitat Politècnica de Catalunya. Departament d'Enginyeria Minera i Recursos Naturals; Universitat Politècnica de Catalunya. TRAGASOL - Grup de Tractament Biològic de Contaminants Gasosos i Olors |
Abstract:
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A novel microelectrode array sensor was fabricated using MEMS technology on a needle, and then applied to real-time measurement of dissolved oxygen (DO) inside biofilms. The sensor consists of eleven gold disk microelectrodes and a rectangular auxiliary electrode along them and an external & internal reference electrode. Three kinds of sensors were designed and their responses were characterized and evaluated under various environmental conditions. The arrays exhibit a linear response to DO in the 0 - 8 mg L-1 concentration range in water, high sensitivity, repeatability, and low limits of detection (< 0.11 mg L-1) and quantification (0.38 mg L-1). The sensors were then validated against a commercial Clark-type microelectrode and applied to profiling of DO in a heterotrophic biofilm cultivated in a flat-plate bioreactor. It is shown that the sensor array can provide a multipoint, simultaneous snapshot profile of DO inside a biofilm with high spatial resolution due to its micrometric dimensions. We conclude that this new sensor array represents a powerful tool for sensing of DO biofilms and in numerous bioprocesses. |
Abstract:
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Peer Reviewed |
Materia(s):
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-Àrees temàtiques de la UPC::Enginyeria química::Química del medi ambient -Àrees temàtiques de la UPC::Enginyeria química::Química analítica -Dissolved Oxygen -Microelectrode Array -Sensors -Bioprocess Monitoring -Biofilms |
Derechos:
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Tipo de documento:
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Artículo - Versión presentada Artículo |
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