Título:
|
A new spiral microelectrode assembly for electroporation and impedance measurements of adherent cell monolayers
|
Autor/a:
|
García Sánchez, Tomás; Guitart, Maria; Rosell Ferrer, Francisco Javier; Gomez Foix, Anna Maria; Bragós Bardia, Ramon
|
Otros autores:
|
Universitat Politècnica de Catalunya. Departament d'Enginyeria Electrònica; Universitat Politècnica de Catalunya. IEB - Instrumentació Electrònica i Biomèdica |
Abstract:
|
In this study, a new microelectrode assembly based on spiral geometry applicable to in situ electroporation of adherent cell monolayers on standard multiwell plates is presented. Furthermore, the structure is specially conceived to perform electrical impedance spectroscopy (EIS) measurements during electroporation. Its performance for cell membrane permeabilization is tested with a fluorescent probe. Gene electrotransfer is also assayed using a plasmid DNA encoding GFP in four different cell lines (CHO, HEK293, 3T3-L1 and FTO2B). Additionally, siRNA alpha-GFP electrotransfection is tested in GFP gene-expressing CHO cells. Our data show considerable differences between permeabilization and gene transfer results and cell line dependence on gene expression rates. Successful siRNA electro-mediated delivery is also achieved. We demonstrate the applicability of our device for electroporation-mediated gene transfer of adherent cells in standard laboratory conditions. Finally, electrical impedance measurements during electroporation of CHO and 3T3-L1 cells are also given. |
Materia(s):
|
-Àrees temàtiques de la UPC::Enginyeria biomèdica::Electrònica biomèdica -Biomedical engineering. -In situ electroporation -Gene electrotransfer -Microelectrodes -Adherent cell -siRNA delivery -Electrical impedance spectroscopy -ELECTROTRANSFER IN-VITRO -LOW-VOLTAGE PULSES -GENE-TRANSFER -MAMMALIAN-CELL -TISSUE ELECTROPORATION -SITU ELECTROPORATION -DNA ELECTROTRANSFER -ELECTRIC PULSES -EXPRESSION -ELECTROPERMEABILIZATION -Enginyeria biomèdica |
Derechos:
|
|
Tipo de documento:
|
Artículo - Versión publicada Artículo |
Compartir:
|
|