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Incorporation of a clot-binding peptide into polythiophene: properties of composites for biomedical applications
Fabregat Jové, Georgina; Cruz Teixeira Dias, Bruno José da; Valle Mendoza, Luis Javier del; Armelín Diggroc, Elaine Aparecida; Estrany Coda, Francesc; Alemán Llansó, Carlos
Universitat Politècnica de Catalunya. Departament d'Enginyeria Química; Universitat Politècnica de Catalunya. IMEM - Innovació, Modelització i Enginyeria en (BIO) Materials; Universitat Politècnica de Catalunya. PSEP - Polimers Sintètics: Estructura i Propietats. Polimers Biodegradables.
Biocomposites formed by a pentapeptide (CREKA), which recognizes clotted plasma proteins, entrapped into the poly(3,4-ethylenedioxythiophene) (PEDOT) matrix have been prepared using three very different procedures. X-ray photoelectron spectroscopy analyses indicate that PEDOT-CREKA films, prepared by chronoamperometry in basic aqueous solution (pH = 10.3) and deposited onto a PEDOT internal layer, present the higher concentration of peptide: one CREKA molecule per six polymer repeat units. The surface of this bilayered system shows numerous folds homogeneously distributed, which have been exhaustively characterized by scanning electron microscopy and atomic force microscopy. Indeed, the morphology and topography of such bilayered films is completely different from those of biocomposite-prepared acid aqueous and organic solutions as polymerization media. The impact of the entrapped peptide molecules in the electrochemical properties of the conducting polymer has been found to be practically negligible. In contrast, biocompatibility assays with two different cellular lines indicate that PEDOT-CREKA favors cellular proliferation, which has been attributed to the binding of the peptide to the fibrin molecules from the serum used as a supplement in the culture medium. The latter assumption has been corroborated examining the ability of PEDOT-CREKA to bind fibrin. The latter ability has been also used to explore an alternative strategy based on the treatment of PEDOT-CREKA with fibrin to promote cell attachment and growth. Overall, the results suggest that PEDOT-CREKA is appropriated for multiple biomedical applications combining the electrochemical properties of conducting polymer and the ability of the peptide to recognize and bind proteins.
Peer Reviewed
Àrees temàtiques de la UPC::Enginyeria química
Peptides
Biomedical engineering
cell proliferation
composite
electroactive polymer
fibrin
peptide
RAY PHOTOELECTRON-SPECTROSCOPY
CONDUCTING POLYMER
POLY(3
4-ETHYLENEDIOXYTHIOPHENE) FILMS
X-RAY
ELECTROCHEMICAL SYNTHESIS
MULTILAYERED FILMS
CELL ATTACHMENT
NEURAL PROBES
IN-VIVO
POLYPYRROLE
Pèptids
Enginyeria biomèdica
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/publishedVersion
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