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ICO amplicon NGS data analysis: a web tool for variant detection in common high-risk hereditary cancer genes analyzed by amplicon GS junior next-Generation Sequencing
López Dóriga, Adriana; Feliubadalo, Lidia; Menendez, Maria Dolores; Lopez Doriga, Sergio; Morón Duran, Francisco D.; del Valle, Jesús; Tornero, Eva; Montes, Eva; Cuesta, Raquel; Campos, Olga; Gómez, Carolina; Pineda, Marta; González, Sara; Moreno, Victor; Capellá Frau, Gabriel José; Lázaro, Conxi
Universitat Politècnica de Catalunya. Departament d'Enginyeria Electrònica; Universitat Politècnica de Catalunya. TIEG - Grup d'Electrònica Industrial Terrassa
Next-generation sequencing (NGS) has revolutionized genomic research and is set to have a major impact on genetic diagnostics thanks to the advent of benchtop sequencers and flexible kits for targeted libraries. Among the main hurdles in NGS are the difficulty of performing bioinformatic analysis of the huge volume of data generated and the high number of false positive calls that could be obtained, depending on the NGS technology and the analysis pipeline. Here, we present the development of a free and user-friendly Web data analysis tool that detects and filters sequence variants, provides coverage information, and allows the user to customize some basic parameters. The tool has been developed to provide accurate genetic analysis of targeted sequencing of common high-risk hereditary cancer genes using amplicon libraries run in a GS Junior System. The Web resource is linked to our own mutation database, to assist in the clinical classification of identified variants. We believe that this tool will greatly facilitate the use of the NGS approach in routine laboratories.
Peer Reviewed
Àrees temàtiques de la UPC::Ciències de la salut::Medicina
Àrees temàtiques de la UPC::Informàtica::Aplicacions de la informàtica::Bioinformàtica
Bioinformatics
Mutation (Biology)
Cancer--Genetic aspects
next generation sequencing
mutation analysis
bioinformatic analysis
variant identification
hereditary cancer
DIAGNOSIS
Bioinformàtica
Mutació (Biologia)
Càncer--Aspectes genètics
info:eu-repo/semantics/publishedVersion
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