Use this identifier to quote or link this document: http://hdl.handle.net/2072/230243

Cholesterol regulates Syntaxin 6 trafficking at the TGN-endosomal boundaries
Reverter Martín, Meritxell; Rentero Alfonso, Carles; Garcia-Melero, A.; Hoque, M.; Vilà de Muga, Sandra; Alvarez-Guaita, A.; Conway, J.; Wood, P.; Cairns, R.; Lykopoulou, L.; Grinberg Vaisman, Daniel Raul; Vilageliu i Arqués, Lluïsa; Bosch, Marta; Heeren, J.; Blasi Cabús, Joan; Timpson, P.; Pol i Sorolla, Albert; Tebar Ramon, Francesc; Murray, RZ.; Grewal, Thomas
Inhibition of cholesterol export from late endosomes causes cellular cholesterol imbalance, including cholesterol depletion in the trans-Golgi network (TGN). Here, using Chinese hamster ovary (CHO) Niemann-Pick type C1 (NPC1) mutant cell lines and human NPC1 mutant fibroblasts, we show that altered cholesterol levels at the TGN/endosome boundaries trigger Syntaxin 6 (Stx6) accumulation into VAMP3, transferrin, and Rab11-positive recycling endosomes (REs). This increases Stx6/VAMP3 interaction and interferes with the recycling of αVβ3 and α5β1 integrins and cell migration, possibly in a Stx6-dependent manner. In NPC1 mutant cells, restoration of cholesterol levels in the TGN, but not inhibition of VAMP3, restores the steady-state localization of Stx6 in the TGN. Furthermore, elevation of RE cholesterol is associated with increased amounts of Stx6 in RE. Hence, the fine-tuning of cholesterol levels at the TGN-RE boundaries together with a subset of cholesterol-sensitive SNARE proteins may play a regulatory role in cell migration and invasion.
2014-05-23
Colesterol
Transport biològic
Biologia molecular
Aparell de Golgi
Cholesterol
Biological transport
Molecular biology
Golgi apparatus
cc-by (c) Reverter Martín, Meritxell et al., 2014
http://creativecommons.org/licenses/by/3.0/es
Article
info:eu-repo/semantics/publishedVersion
Elsevier
         

Show full item record

Related documents

Other documents of the same author

Reverter Martín, Meritxell; Rentero Alfonso, Carles; Garcia-Melero, A.; Hoque, M.; Vilà de Muga, Sandra; Alvarez-Guaita, Anna; Conway, J.; Wood, Peta; Cairns, R.; Lykopoulou, L.; Grinberg Vaisman, Daniel Raúl; Vilageliu i Arqués, Lluïsa; Bosch, Marta (Bosch Rodríguez); Heeren, J.; Blasi Cabús, Joan; Timpson, Paul; Pol i Sorolla, Albert; Tebar Ramon, Francesc; Murray, RZ.; Grewal, Thomas
Reverter Martín, Meritxell; Rentero Alfonso, Carles; Garcia-Melero, A.; Hoque, M.; Vilà de Muga, Sandra; Alvarez-Guaita, Anna; Conway, J.; Wood, Peta; Cairns, R.; Lykopoulou, L.; Grinberg Vaisman, Daniel Raúl; Vilageliu i Arqués, Lluïsa; Bosch, Marta (Bosch Rodríguez); Heeren, J.; Blasi Cabús, Joan; Timpson, Paul; Pol i Sorolla, Albert; Tebar Ramon, Francesc; Murray, RZ.; Grewal, Thomas
Reverter Martín, Meritxell; Rentero Alfonso, Carles; Garcia-Melero, A.; Hoque, M.; Vilà de Muga, Sandra; Alvarez-Guaita, Anna; Conway, J.; Wood, Peta; Cairns, R.; Lykopoulou, L.; Grinberg Vaisman, Daniel Raúl; Vilageliu i Arqués, Lluïsa; Bosch, Marta (Bosch Rodríguez); Heeren, J.; Blasi Cabús, Joan; Timpson, Paul; Pol i Sorolla, Albert; Tebar Ramon, Francesc; Murray, RZ.; Grewal, Thomas
Reverter Martín, Meritxell; Rentero Alfonso, Carles; Vilà de Muga, Sandra; Alvarez-Guaita, Anna; Mulay, Vishwaroop; Wood, Peta; Monastyrskaya, Katia; Pol i Sorolla, Albert; Tebar Ramon, Francesc; Blasi Cabús, Joan; Grewal, Thomas; Enrich Bastús, Carles
Reverter Martín, Meritxell; Rentero Alfonso, Carles; Vilà de Muga, Sandra; Alvarez-Guaita, Anna; Mulay, Vishwaroop; Wood, Peta; Monastyrskaya, Katia; Pol i Sorolla, Albert; Tebar Ramon, Francesc; Blasi Cabús, Joan; Grewal, Thomas; Enrich Bastús, Carles
 

Coordination

 

Supporters