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Lysine-based surfactants in nanovesicle formulations: the role of cationic charge position and hydrophobicity in in vitro cytotoxicity and intracellular delivery
Nogueira, Daniele R.; Morán Badenas, María del Carmen; Mitjans Arnal, Montserrat; Pérez Muñoz, Lourdes; Ramos, David; Lapuente Pérez, Joaquín de; Vinardell Martínez-Hidalgo, Ma. Pilar
Universitat de Barcelona
Understanding nanomaterial interactions within cells is of increasing importance for assessing their toxicity and cellular transport. Here, we developed nanovesicles containing bioactive cationic lysine-based amphiphiles, and assessed whether these cationic compounds increase the likelihood of intracellular delivery and modulate toxicity. We found different cytotoxic responses among the formulations, depending on surfactant, cell line and endpoint assayed. The induction of mitochondrial dysfunction, oxidative stress and apoptosis were the general mechanisms underlying cytotoxicity. Fluorescence microscopy analysis demonstrated that nanovesicles were internalized by HeLa cells, and evidenced that their ability to release endocytosed materials into cell cytoplasm depends on the structural parameters of amphiphiles. The cationic charge position and hydrophobicity of surfactants determine the nanovesicle interactions within the cell and, thus, the resulting toxicity and intracellular behavior after cell uptake of the nanomaterial. The insights into some toxicity mechanisms of these new nanomaterials contribute to reducing the uncertainty surrounding their potential health hazards.
Toxicologia
Materials nanoestructurats
Sistemes d'alliberament de medicaments
Cultiu cel·lular
Citotoxicitat per mediació cel·lular
Toxicology
Nanostructured materials
Drug delivery systems
Cell culture
Cell-mediated cytotoxicity
(c) Informa Healthcare, 2013
Article
info:eu-repo/semantics/acceptedVersion
Informa Healthcare
         

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