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Kinesin-5/Eg5 is important for transport of CARTS from the trans-Golgi network to the cell surface
Wakana, Yuichi; Villeneuve, Julien; Galen, Josse van; Cruz Garcia, David; Tagaya, Mitsuo; Malhotra, Vivek
Here we report that the kinesin-5 motor Klp61F, which is known for its role in bipolar spindle formation in mitosis, is required for protein transport from the Golgi complex to the cell surface in Drosophila S2 cells. Disrupting the function of its mammalian orthologue, Eg5, in HeLa cells inhibited secretion of a protein called pancreatic adenocarcinoma up-regulated factor (PAUF) but, surprisingly, not the trafficking of vesicular stomatitis virus G protein (VSV-G) to the cell surface. We have previously reported that PAUF is transported from the trans-Golgi network (TGN) to the cell surface in specific carriers called CARTS that exclude VSV-G. Inhibition of Eg5 function did not affect the biogenesis of CARTS; however, their migration was delayed and they accumulated near the Golgi complex. Altogether, our findings reveal a surprising new role of Eg5 in nonmitotic cells in the facilitation of the transport of specific carriers, CARTS, from the TGN to the cell surface.
J. Villeneuve is funded by a long-term EMBO postdoctoral fellowship. V. Malhotra is an Institució Catalana de Recerca i Estudis Avançats (ICREA) professor at the Centre for Genomic Regulation, and the work in his laboratory is funded by grants from Plan Nacional (BFU2008-00414), Consolider (CSD2009-00016), and European Research Council (268692)
12-05-2014
Proteïnes
Aparell de Golgi
© Yuichi Wakana et al., 2013. This article is distributed under aCreative Commons License Attribution–Noncommercial–Share Alike 3.0 Unported.
http://creativecommons.org/licenses/by/3.0/
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info:eu-repo/semantics/publishedVersion
Rockefeller University Press
         

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