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Biselectivity of isoDGR peptides for fibronectin binding integrin subtypes a5ß1 and avß6: conformational control through flanking amino acids
Bochen, Alexander; Marelli, Udaya Kiran; Otto, Elke; Pallarola, Diego; Mas Moruno, Carlos; Di Leva, Francisco Saverio; Boehm, Heike; Spatz, Joaquim P.; Novellino, Ettore; Kessler, Horst; Marinelli, Luciana
Universitat Politècnica de Catalunya. Departament de Ciència dels Materials i Enginyeria Metal·lúrgica
Integrins are the major class of cell adhesion proteins. Their interaction with different ligands of the extracellular matrix is diverse. To get more insight into these interactions, artificial ligands endowed with a well-defined activity/selectivity profile are necessary. Herein, we present a library of cyclic pentapeptides, based on our previously reported peptide motif c(-phg-isoDGR-X-), in which high activity toward fibronectin binding integrins a5ß1 and avß6 and not on vitronectin binding integrins avß3 and avß5 has been achieved by changing the flanking amino acids. The structure of the most promising candidates has been determined using a combined approach of NMR, distance geometry, and molecular dynamics simulations, and docking studies have been further used to elucidate the peptide–integrin interactions at the molecular level. The peptides’ binding affinity has been characterized by enzyme linked immunosorbent assay experiments, and the results have been verified by cell adhesion experiments on specifically functionalized surfaces
Peer Reviewed
Àrees temàtiques de la UPC::Enginyeria dels materials
Attribution-NonCommercial-NoDerivs 3.0 Spain

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