Para acceder a los documentos con el texto completo, por favor, siga el siguiente enlace: http://hdl.handle.net/2445/50983

Undifferentiated and differentiated PC12 cells protected by huprines against injury induced by hydrogen peroxide
Pera, Marta; Camps García, Pelayo; Muñoz-Torrero López-Ibarra, Diego; Pérez Fernández, Belén; Badía, A. (Albert); Clos Guillén, M. Victòria
Universitat de Barcelona
Oxidative stress is implicated in the pathogenesis of neurodegenerative disorders and hydrogen peroxide (H2O2) plays a central role in the stress. Huprines, a group of potent acetylcholinesterase inhibitors (AChEIs), have shown a broad cholinergic pharmacological profile. Recently, it has been observed that huprine X (HX) improves cognition in non transgenic middle aged mice and shows a neuroprotective activity (increased synaptophysin expression) in 3xTg-AD mice. Consequently, in the present experiments the potential neuroprotective effect of huprines (HX, HY, HZ) has been analyzed in two different in vitro conditions: undifferentiated and NGF-differentiated PC12 cells. Cells were subjected to oxidative insult (H2O2, 200 µM) and the protective effects of HX, HY and HZ (0.01 µM- 1 µM) were analyzed after a pre-incubation period of 24 and 48 hours. All huprines showed protective effects in both undifferentiated and NGF-differentiated cells, however only in differentiated cells the effect was dependent on cholinergic receptors as atropine (muscarinic antagonist, 0.1 µM) and mecamylamine (nicotinic antagonist, 100 µM) reverted the neuroprotection action of huprines. The decrease in SOD activity observed after oxidative insult was overcome in the presence of huprines and this effect was not mediated by muscarinic or nicotinic receptors. In conclusion, huprines displayed neuroprotective properties as previously observed in in vivo studies. In addition, these effects were mediated by cholinergic receptors only in differentiated cells. However, a non-cholinergic mechanism, probably through an increase in SOD activity, seems to be also involved in the neuroprotective effects of huprines.
Inhibidors enzimàtics
Acetilcolinesterasa
Malalties neurodegeneratives
Aigua oxigenada
Enzyme inhibitors
Acetylcholinesterase
Neurodegenerative Diseases
Hydrogen peroxide
cc-by (c) Pera, Marta et al., 2013
http://creativecommons.org/licenses/by/3.0/es
Artículo
info:eu-repo/semantics/publishedVersion
Public Library of Science (PLoS)
         

Mostrar el registro completo del ítem

Documentos relacionados

Otros documentos del mismo autor/a

Di Pietro, O.; Viayna, E.; Vicente García, Esther; Bartolini, Manuela; Ramón, Rosario; Juárez-Jiménez, Jordi; Clos Guillén, M. Victòria; Pérez Fernández, Belén; Andrisano, Vincenza; Luque Garriga, F. Xavier; Lavilla Grífols, Rodolfo; Muñoz-Torrero López-Ibarra, Diego
Di Pietro, O.; Viayna, E.; Vicente García, Esther; Bartolini, Manuela; Ramón, Rosario; Juárez-Jiménez, Jordi; Clos Guillén, M. Victòria; Pérez Fernández, Belén; Andrisano, Vincenza; Luque Garriga, F. Xavier; Lavilla Grífols, Rodolfo; Muñoz-Torrero López-Ibarra, Diego
Di Pietro, O.; Vicente García, Esther; Taylor, Martin C.; Berenguer, Diana; Viayna, Elisabet; Lanzoni, Anna; Sola, Irene; Sayago, Helena; Riera Lizandra, Ma. Cristina; Fisa Saladrigas, Roser; Clos Guillén, M. Victòria; Pérez Fernández, Belén; Kelly, John M.; Lavilla Grífols, Rodolfo; Muñoz-Torrero López-Ibarra, Diego
Di Pietro, O.; Viayna, Elisabet; Vicente García, Esther; Bartolini, Manuela; Ramón, Rosario; Juárez-Jiménez, Jordi; Clos Guillén, M. Victòria; Pérez Fernández, Belén; Andrisano, Vincenza; Luque Garriga, F. Xavier; Lavilla Grífols, Rodolfo; Muñoz-Torrero López-Ibarra, Diego
Viayna, Elisabet; Sola, Irene; Bartolini, Manuela; De Simone, A.; Tapia-Rojas, C.; Serrano, Felipe G.; Sabaté Lagunas, Raimon; Juárez-Jiménez, Jordi; Pérez Fernández, Belén; Luque Garriga, F. Xavier; Andrisano, Vincenza; Clos Guillén, M. Victòria; Inestrosa, Nibaldo C.; Muñoz-Torrero López-Ibarra, Diego